Exercise rescues the immune response fine-tuned impaired by peroxisome proliferator-activated receptors deletion in macrophages

Autor(es)

Silveira, Loreana Sanches ^{[1, 2]} ; Pereira Batatinha, Helena Angelica ^[2] ; Castoldi, Angela ^[3] ; Saraiva Camara, Niels Olsen ^[3] ; Festuccia, Willian T. ^[4] ; Souza, Camila Oliveira ^[2] ; Rosa Neto, Jose Cesar ^[2] ; Lira, Fabio Santos ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Post Grad Program Movement Sci, Exercise & Immunometab Res Grp, Dept Phys Educ, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Av Lineu Prestes 1524, Lab 435, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. 234, n. 4, p. 5241-5251, APR 2019.
• Citações Web of Science: 1

Resumo

BackgroundExercise is a powerful tool for prevention and treatment of many conditions related to the cardiovascular system and also chronic low-grade inflammation. Peroxisome proliferator-activated receptors (PPAR) exerts an import role on the regulation of metabolic profile and subsequent inflammatory response, especially in macrophages. PurposeTo investigate the effects of 8-week moderate-exercise training on metabolic and inflammatory parameters in mice with PPAR deficiency in myeloid cells. MethodsTwelve-week old mice bearing PPAR deletion exclusively in myeloid cells (PPARlox/lox Lys Cre (-/+), knockout {[}KO]) and littermate controls (PPARlox/lox Lys Cre (-/-), wild type {[}WT]) were submitted to 8-week exercise training (treadmill running at moderate intensity, 5 days/week). Animals were evaluated for food intake, glucose homeostasis, serum metabolites, adipose tissue and peritoneal macrophage inflammation, and basal and stimulated cytokine secretion. ResultsExercise protocol did not improve glucose metabolism or adiponectin concentrations in serum of KO mice. Moreover, the absence of PPAR in macrophages exacerbated the proinflammatory profile in sedentary mice. Peritoneal cultured cells had higher tumor necrosis factor- (TNF-) secretion in nonstimulated and lipopolysaccharide (LPS)-stimulated conditions and higher Toll-4 receptor (TLR4) gene expression under LPS stimulus. Trained mice showed reduced TNF- content in adipose tissue independently of the genotype. M2 polarization ability was impaired in KO peritoneal macrophages after exercise training, while adipose tissue-associated macrophages did not present any effect by PPAR ablation. ConclusionOverall, PPAR seems necessary to maintain macrophages appropriate response to inflammatory stimulus and macrophage polarization, affecting also whole body lipid metabolism and adiponectin profile. Exercise training showed as an efficient mechanism to restore the immune response impaired by PPAR deletion in macrophages. (AU)

• Processo FAPESP: 14/01246-6 - Avaliação da resposta imuno-metabólica em macrófagos peritoneais de camundongos obesos: papel do exercício físico e do PPAR-gamma
• Beneficiário: Loreana Sanches Silveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/01409-8 - Caracterização do efeito antiinflamatório da suplementação com ácido palmitoleico na inflamação hepática; o papel dos ppars.
• Beneficiário: José Cesar Rosa Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/25310-2 - Avaliação da resposta imuno-metabólica em células sanguíneas em indivíduos obesos: papel do exercício físico e do PPAR-gama
• Beneficiário: Fábio Santos de Lira
• Modalidade de apoio: Auxílio à Pesquisa - Regular