Laminar shear stress-provoked cytoskeletal changes are mediated by epigenetic reprogramming of TIMP1 in human primary smooth muscle cells

Autor(es)

da Silva, Rodrigo A. ^[1] ; Fernandes, Celio Jr da C. ; Feltran, Georgia da S. ^[2] ; Gomes, Anderson M. ^[2] ; de Camargo Andrade, Amanda Fantini ^[2] ; Andia, Denise C. ^[3] ; Peppelenbosch, Maikel P. ^[4] ; Zambuzzi, Willian F. ^{[2, 5]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Lab Bioassays & Cellular Dynam, Dept Chem & Biochem, Campus Botucatu, Botucatu, SP - Brazil

^[2] Fernandes, Jr., Celio Jr da C., Sao Paulo State Univ UNESP, Inst Biosci, Lab Bioassays & Cellular Dynam, Dept Chem & Biochem, Campus Botucatu, Botucatu, SP - Brazil

^[3] Univ Paulista, UNIP, Fac Odontol, Area Pesquisa Epigenet, Sao Paulo, SP - Brazil

^[4] Erasmus MC, Univ Med Ctr Rotterdam, Dept Gastroenterol & Hepatol, Rotterdam - Netherlands

^[5] Sao Paulo State Univ UNESP, Inst Biosci, Electron Microscopy Ctr, Campus Botucatu, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. 234, n. 5, p. 6382-6396, MAY 2019.
• Citações Web of Science: 5

Resumo

Whereas endothelial responses to shear stress are well-characterized, the cell physiological effects of shear stress in smooth muscle cells (SMCs) remain largely obscure. As SMCs are directly challenged by shear stress after endothelial denuding injury following procedures such as angioplasty or endarterectomy, characterization of these responses represents an important scientific question. Hence we decided to contrast cytoskeletal reorganization, epigenetic reprogramming, signaling transduction, and changes in miRNA (miRs) profiles in primary human aortic smooth muscle cells (AoSMCs) between unstressed cells and cells exposed to shear stress. We observed that shear stress-provoked reorganization of the actin cytoskeleton in an apparently Cofilin-dependent fashion and which related to altered integrin signaling, apparently caused by remodeling of the extracellular matrix. The latter appeared a downstream effect of increased expression of matrix metalloproteinases and downregulation of tissue metalloproteinase inhibitor 1 (TIMP1) protein levels. In turn, these effects related to shear stress-provoked changes in expression and nuclear localization of the epigenetic regulators demethylases TET1, TET2, DNMT1, DNMT3A and DNMT3B, HDAC6, and SIRT1. Accordingly, TIMP1 promotor CpG hypomethylation was a prominent effect, and resulted in a significant increase in TIMP1 transcription, which may also have related increased expression of miRs involved in modulating TIMP1 translation. Thus epigenetic-reprogramming of TIMP1 emerges as critical element in smooth muscle responses to mechanical signals and as epigenetic machinery is amendable to pharmacological manipulation, this pathway may have important clinical consequences. (AU)

• Processo FAPESP: 16/01139-0 - Regulação epigenética mediada por fatores parácrinos produzidos por células endoteliais em células osteoblásticas
• Beneficiário: Rodrigo Augusto da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/22689-3 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo
• Beneficiário: Willian Fernando Zambuzzi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/08888-9 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo
• Beneficiário: Célio Junior da Costa Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto