De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production

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Batista, Diego Silva ^[1] ; Koehler, Andrea Dias ^[2] ; Romanel, Elisson ^[3] ; de Souza, Vinicius Cairus ^[4] ; Silva, Tatiane Dulcineia ^[2] ; Almeida, Maira Carolina ^[2] ; Maciel, Talles Elisson F. ^[5] ; Bueno Ferreira, Peracio Rafael ^[2] ; Sousa Felipe, Sergio Heitor ^[2] ; Saldanha, Cleber Witt ^[6] ; Maldaner, Joseila ^[6] ; Carnevalli Dias, Leonardo Lucas ^[7] ; Festucci-Buselli, Reginaldo Alves ^[8] ; Otoni, Wagner Campos ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Maranhao, Sao Luis, MA - Brazil

^[2] Univ Fed Vicosa, Dept Biol Vegetal, BIOAGRO, Vicosa, MG - Brazil

^[3] Univ Sao Paulo, Lab Genom Plantas & Bioenergia PGEMBL, Dept Biotecnol, EEL, Lorena, SP - Brazil

^[4] Univ Fed Juiz de Fora, Dept Ciencia Comp, Juiz De Fora, MG - Brazil

^[5] Univ Fed Vicosa, Dept Bioquim & Biol Mol, Vicosa, MG - Brazil

^[6] SEAPI, Ctr Pesquisa Florestas, DDPA, Santa Maria, RS - Brazil

^[7] Univ Fed Sao Joao del Rei, Campus Sete Lagoas, Sete Lagoas, MG - Brazil

^[8] Univ Fed Rural Amazonia, Lab Bioquim & Genet Mol, Belem, PA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PROTOPLASMA; v. 256, n. 3, p. 601-614, MAY 2019.
• Citações Web of Science: 3

Resumo

Pfaffia glomerata is a medically important species because it produces the phytoecdysteroid 20-hydroxyecdysone (20-E). However, there has been no ready-to-use transcriptome data available in the literature for this plant. Here, we present de novo transcriptome sequencing of RNA from P. glomerata in order to investigate the 20-E production as well as to understand the biochemical pathway of secondary metabolites in this non-model species. We then analyze the effect of photoautotrophy on the production of 20-E genes phylogenetically identified followed by expression analysis. For this, total messenger RNA (mRNA) from leaves, stems, roots, and flowers was used to construct indexed mRNA libraries. Based on the similarity searches against plant non-redundant protein database, gene ontology, and eukaryotic orthologous groups, 164,439 transcripts were annotated. In addition, the effect of photoautotrophy in two genes putatively involved in the 20-E synthesis pathway was analyzed. The Phantom gene (CYP76C), a precursor of the route, showed increased expression in P. glomerata plants cultured under photoautotrophic conditions. This was accompanied by increased production of this metabolite indicating a putative involvement in 20-E synthesis. This work reveals that several genes in the P. glomerata transcriptome are related to secondary metabolism and stresses, that genes of the P450 family participate in the 20-E biosynthesis route, and that plants cultured under photoautotrophic conditions promote an upregulated Phantom gene and enhance the productivity of 20-E. The data will be used for future investigations of the 20-E synthesis pathway in P. glomerata while offering a better understanding of the metabolism of the species. (AU)

• Processo FAPESP: 14/06923-6 - Recalcitrância da biomassa de cana-de-açúcar: fundamentos relacionados à formação da parede celular, ao pré-tratamento e à digestão enzimática, aplicados no desenvolvimento de novos modelos de biorrefinarias
• Beneficiário: Andre Luis Ferraz
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 14/17486-6 - Genômica da biossíntese de hemicelulose em cana-de-açúcar: visando compreender a relação dos genes com a recalcitrância da parede celular
• Beneficiário: Elisson Antonio da Costa Romanel
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular