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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production

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Author(s):
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Batista, Diego Silva [1] ; Koehler, Andrea Dias [2] ; Romanel, Elisson [3] ; de Souza, Vinicius Cairus [4] ; Silva, Tatiane Dulcineia [2] ; Almeida, Maira Carolina [2] ; Maciel, Talles Elisson F. [5] ; Bueno Ferreira, Peracio Rafael [2] ; Sousa Felipe, Sergio Heitor [2] ; Saldanha, Cleber Witt [6] ; Maldaner, Joseila [6] ; Carnevalli Dias, Leonardo Lucas [7] ; Festucci-Buselli, Reginaldo Alves [8] ; Otoni, Wagner Campos [2]
Total Authors: 14
Affiliation:
[1] Univ Estadual Maranhao, Sao Luis, MA - Brazil
[2] Univ Fed Vicosa, Dept Biol Vegetal, BIOAGRO, Vicosa, MG - Brazil
[3] Univ Sao Paulo, Lab Genom Plantas & Bioenergia PGEMBL, Dept Biotecnol, EEL, Lorena, SP - Brazil
[4] Univ Fed Juiz de Fora, Dept Ciencia Comp, Juiz De Fora, MG - Brazil
[5] Univ Fed Vicosa, Dept Bioquim & Biol Mol, Vicosa, MG - Brazil
[6] SEAPI, Ctr Pesquisa Florestas, DDPA, Santa Maria, RS - Brazil
[7] Univ Fed Sao Joao del Rei, Campus Sete Lagoas, Sete Lagoas, MG - Brazil
[8] Univ Fed Rural Amazonia, Lab Bioquim & Genet Mol, Belem, PA - Brazil
Total Affiliations: 8
Document type: Journal article
Source: PROTOPLASMA; v. 256, n. 3, p. 601-614, MAY 2019.
Web of Science Citations: 3
Abstract

Pfaffia glomerata is a medically important species because it produces the phytoecdysteroid 20-hydroxyecdysone (20-E). However, there has been no ready-to-use transcriptome data available in the literature for this plant. Here, we present de novo transcriptome sequencing of RNA from P. glomerata in order to investigate the 20-E production as well as to understand the biochemical pathway of secondary metabolites in this non-model species. We then analyze the effect of photoautotrophy on the production of 20-E genes phylogenetically identified followed by expression analysis. For this, total messenger RNA (mRNA) from leaves, stems, roots, and flowers was used to construct indexed mRNA libraries. Based on the similarity searches against plant non-redundant protein database, gene ontology, and eukaryotic orthologous groups, 164,439 transcripts were annotated. In addition, the effect of photoautotrophy in two genes putatively involved in the 20-E synthesis pathway was analyzed. The Phantom gene (CYP76C), a precursor of the route, showed increased expression in P. glomerata plants cultured under photoautotrophic conditions. This was accompanied by increased production of this metabolite indicating a putative involvement in 20-E synthesis. This work reveals that several genes in the P. glomerata transcriptome are related to secondary metabolism and stresses, that genes of the P450 family participate in the 20-E biosynthesis route, and that plants cultured under photoautotrophic conditions promote an upregulated Phantom gene and enhance the productivity of 20-E. The data will be used for future investigations of the 20-E synthesis pathway in P. glomerata while offering a better understanding of the metabolism of the species. (AU)

FAPESP's process: 14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models
Grantee:Andre Luis Ferraz
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 14/17486-6 - Genomics of hemicellulose biosynthesis in sugarcane: understanding the relationship of genes and the recalcitrance of cell wall
Grantee:Elisson Antonio da Costa Romanel
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants