Sex differences in subcutaneous adipose tissue redox homeostasis and inflammation markers in control and high-fat diet fed rats

Autor(es)

Vasconcelos, Renata Prado ^[1] ; Peixoto, Milena Simoes ^[2] ; de Oliveira, Keciany Alves ^[1] ; Freitas Ferreira, Andrea Claudia ^[3] ; Coelho-de-Souza, Andrelina Noronha ^[1] ; Carvalho, Denise P. ^[3] ; de Oliveira, Ariclecio Cunha ^{[1, 3]} ; Fortunato, Rodrigo S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Ceara, Inst Super Ciencias Biomed, Lab Fisiol Endocrina & Metab, BR-60714903 Fortaleza, Ceara - Brazil

^[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisiol & Sinalizacao Redox, BR-21941902 Rio De Janeiro - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisiol Endocrina Doris Rosenthal, BR-21941902 Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: APPLIED PHYSIOLOGY NUTRITION AND METABOLISM; v. 44, n. 7, p. 720-726, JUL 2019.
• Citações Web of Science: 1

Resumo

The development of obesity-related metabolic disorders is more evident in male in comparison with female subjects, but the mechanisms are unknown. Several studies have shown that oxidative stress is involved in the pathophysiology of obesity, but the majority of these studies were performed with male animals. The aim of this study was to evaluate the sex-related differences in subcutaneous adipose tissue redox homeostasis and inflammation of rats chronically fed a high-fat diet. NADPH oxidase (NOX), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase activities were evaluated in the subcutaneous adipose tissue (SC) of adult male and female rats fed either a standard chow (SCD) or a high-fat diet (HFD) for 11 weeks. NOX2 and NOX4 messenger RNA (mRNA) levels, total reduced thiols, interleukin (IL)-1 beta, tumor necrosis factor alpha (TNF-alpha), and IL-6 were also determined. Higher antioxidant enzyme activities and total reduced thiol levels were detected in SC of control male compared with female rats. Chronic HFD administration increased NOX activity and NOX2 and NOX4 mRNA levels and decreased SOD and GPx activities only in male animals. IL-1 beta, TNF-alpha, and IL-6 levels, as well as Adgre1, CD11b, and CD68 mRNA levels, were also higher in SC of males after HFD feeding. In SC of females, catalase activity was higher after HFD feeding. Taken together, our results show that redox homeostasis and inflammation of SC is sexually dimorphic. Furthermore, males show higher oxidative stress in SC after 11 weeks of HFD feeding owing to both increased reactive oxygen species (ROS) production through NOX2 and NOX4 and decreased ROS detoxification. (AU)

• Processo FAPESP: 14/15982-6 - Consequências de deficiências de reparo de lesões no genoma
• Beneficiário: Carlos Frederico Martins Menck
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/21075-9 - Participação da NADPH oxidase 2 induzida pelo estrogênio em lesões no DNA e na regulação da autofagia em linhagem celular de mama
• Beneficiário: Carlos Frederico Martins Menck
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Brasil