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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sex differences in subcutaneous adipose tissue redox homeostasis and inflammation markers in control and high-fat diet fed rats

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Author(s):
Vasconcelos, Renata Prado [1] ; Peixoto, Milena Simoes [2] ; de Oliveira, Keciany Alves [1] ; Freitas Ferreira, Andrea Claudia [3] ; Coelho-de-Souza, Andrelina Noronha [1] ; Carvalho, Denise P. [3] ; de Oliveira, Ariclecio Cunha [1, 3] ; Fortunato, Rodrigo S. [2]
Total Authors: 8
Affiliation:
[1] Univ Estadual Ceara, Inst Super Ciencias Biomed, Lab Fisiol Endocrina & Metab, BR-60714903 Fortaleza, Ceara - Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisiol & Sinalizacao Redox, BR-21941902 Rio De Janeiro - Brazil
[3] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisiol Endocrina Doris Rosenthal, BR-21941902 Rio De Janeiro - Brazil
Total Affiliations: 3
Document type: Journal article
Source: APPLIED PHYSIOLOGY NUTRITION AND METABOLISM; v. 44, n. 7, p. 720-726, JUL 2019.
Web of Science Citations: 1
Abstract

The development of obesity-related metabolic disorders is more evident in male in comparison with female subjects, but the mechanisms are unknown. Several studies have shown that oxidative stress is involved in the pathophysiology of obesity, but the majority of these studies were performed with male animals. The aim of this study was to evaluate the sex-related differences in subcutaneous adipose tissue redox homeostasis and inflammation of rats chronically fed a high-fat diet. NADPH oxidase (NOX), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase activities were evaluated in the subcutaneous adipose tissue (SC) of adult male and female rats fed either a standard chow (SCD) or a high-fat diet (HFD) for 11 weeks. NOX2 and NOX4 messenger RNA (mRNA) levels, total reduced thiols, interleukin (IL)-1 beta, tumor necrosis factor alpha (TNF-alpha), and IL-6 were also determined. Higher antioxidant enzyme activities and total reduced thiol levels were detected in SC of control male compared with female rats. Chronic HFD administration increased NOX activity and NOX2 and NOX4 mRNA levels and decreased SOD and GPx activities only in male animals. IL-1 beta, TNF-alpha, and IL-6 levels, as well as Adgre1, CD11b, and CD68 mRNA levels, were also higher in SC of males after HFD feeding. In SC of females, catalase activity was higher after HFD feeding. Taken together, our results show that redox homeostasis and inflammation of SC is sexually dimorphic. Furthermore, males show higher oxidative stress in SC after 11 weeks of HFD feeding owing to both increased reactive oxygen species (ROS) production through NOX2 and NOX4 and decreased ROS detoxification. (AU)

FAPESP's process: 14/15982-6 - Consequences of repair deficiencies in damaged genome
Grantee:Carlos Frederico Martins Menck
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/21075-9 - Participation of NADPH oxidase 2 induced by estrogen in the formation of DNA damage and autophagy regulation in breast cells
Grantee:Carlos Frederico Martins Menck
Support type: Research Grants - Visiting Researcher Grant - Brazil