iNOS/Arginase-1 expression in the pulmonary tissue over time during Cryptococcus gattii infection

Autor(es)

Martins Oliveira-Brito, Patricia Kellen ^[1] ; Rezende, Caroline Patini ^[2] ; Almeida, Fausto ^[2] ; Roque-Barreira, Maria Cristina ^[1] ; da Silva, Thiago Aparecido ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Innate Immunity; v. 26, n. 2 AUG 2019.
• Citações Web of Science: 0

Resumo

Inhalation of Cryptococcus gattii yeasts (causing cryptococcosis) triggers an anti-cryptococcal immune response initiated by macrophages, neutrophils or dendritic cells, and the iNOS expressed by various cells may regulate the function and differentiation of innate and adaptive immune cells. Here, we evaluated the effect of progression of C. gattii infection on the host innate immune response. C. gattii infection in BALB/c mice spreads to several organs by 21 d post infection. The numbers of neutrophils and lymphocytes in the peripheral blood of C. gattii-infected mice were remarkably altered on that day. The frequency of CD11b(+) cells and cell concentrations of CD4(+) and CD8(+) T cells was significantly altered in the pulmonary tissue of infected mice. We found a higher frequency of CD11b(+)/iNOS(+) cells in the lungs of infected mice, accompanied by an increase in frequency of CD11b(+)/Arginase-1(+) cells over time. Moreover, the iNOS/Arginase-1 expression ratio in CD11b(+) cells reached its lowest value at 21 d post infection. In addition, the cytokine micro-environment in infected lungs did not show a pro-inflammatory profile. Surprisingly, iNOS knock-out prolonged the survival of infected mice, while their pulmonary fungal burden was higher than that of infected WT mice. Thus, C. gattii infection alters the immune response in the pulmonary tissue, and iNOS expression may play a key role in infection progression. (AU)

• Processo FAPESP: 16/03322-7 - Papel de galectina-3 na infecção por Cryptococcus neoformans
• Beneficiário: Fausto Bruno dos Reis Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/23112-7 - A atividade imunomoduladora de ArtinM sobre o curso da infecção por Cryptococcus gattii
• Beneficiário: Patrícia Kellen Martins Oliveira Brito
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/04877-2 - Delineamento de novas estratégias terapêuticas, baseadas em reconhecimento de carboidratos, contra a criptococose
• Beneficiário: Thiago Aparecido da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/10446-4 - Mecanismos da imunomodulação por ArtinM: bases para o desenvolvimento de nova terapia anti-fúngica
• Beneficiário: Maria Cristina Roque Antunes Barreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/25167-3 - Influência de galectina-3 no curso da infecção por Cryptococcus neoformans
• Beneficiário: Caroline Patini de Rezende
• Modalidade de apoio: Bolsas no Brasil - Mestrado