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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

iNOS/Arginase-1 expression in the pulmonary tissue over time during Cryptococcus gattii infection

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Martins Oliveira-Brito, Patricia Kellen [1] ; Rezende, Caroline Patini [2] ; Almeida, Fausto [2] ; Roque-Barreira, Maria Cristina [1] ; da Silva, Thiago Aparecido [1]
Total Authors: 5
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Innate Immunity; v. 26, n. 2 AUG 2019.
Web of Science Citations: 0

Inhalation of Cryptococcus gattii yeasts (causing cryptococcosis) triggers an anti-cryptococcal immune response initiated by macrophages, neutrophils or dendritic cells, and the iNOS expressed by various cells may regulate the function and differentiation of innate and adaptive immune cells. Here, we evaluated the effect of progression of C. gattii infection on the host innate immune response. C. gattii infection in BALB/c mice spreads to several organs by 21 d post infection. The numbers of neutrophils and lymphocytes in the peripheral blood of C. gattii-infected mice were remarkably altered on that day. The frequency of CD11b(+) cells and cell concentrations of CD4(+) and CD8(+) T cells was significantly altered in the pulmonary tissue of infected mice. We found a higher frequency of CD11b(+)/iNOS(+) cells in the lungs of infected mice, accompanied by an increase in frequency of CD11b(+)/Arginase-1(+) cells over time. Moreover, the iNOS/Arginase-1 expression ratio in CD11b(+) cells reached its lowest value at 21 d post infection. In addition, the cytokine micro-environment in infected lungs did not show a pro-inflammatory profile. Surprisingly, iNOS knock-out prolonged the survival of infected mice, while their pulmonary fungal burden was higher than that of infected WT mice. Thus, C. gattii infection alters the immune response in the pulmonary tissue, and iNOS expression may play a key role in infection progression. (AU)

FAPESP's process: 16/03322-7 - Role of galectin-3 in the Cryptococcus neoformans infection
Grantee:Fausto Bruno dos Reis Almeida
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 16/23112-7 - The immunomodulatory activity of ArtinM on the course of Cryptococcus gattii infection
Grantee:Patrícia Kellen Martins Oliveira Brito
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/04877-2 - Design of new therapeutic strategies, based on the carbohydrate recognition, against cryptococcosis
Grantee:Thiago Aparecido da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/10446-4 - Mechanisms of immunomodulation by ArtinM: basis for the development of new anti-fungal therapy
Grantee:Maria Cristina Roque Antunes Barreira
Support type: Regular Research Grants
FAPESP's process: 16/25167-3 - Influence of galectin-3 on course of infection by Cryptococcus neoformans
Grantee:Caroline Patini de Rezende
Support type: Scholarships in Brazil - Master