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Role of galectin-3 in the Cryptococcus neoformans infection

Grant number: 16/03322-7
Support type:Research Grants - Young Investigators Grants
Duration: July 01, 2016 - June 30, 2020
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Fausto Bruno dos Reis Almeida
Grantee:Fausto Bruno dos Reis Almeida
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):16/25167-3 - Influence of galectin-3 on course of infection by Cryptococcus neoformans, BP.MS
16/15055-3 - Role of galectin-3 in the Cryptococcus neoformans infection, BP.JP

Abstract

Cryptococcus neoformans is the causative agent of cryptococcosis and a major pathogen for immunosuppressed individuals. C. neoformans represents a unique model in cell biology studies since it is the only eukaryotic pathogen with a polysaccharide capsule, furthermore produces vesicles containing its major virulence factor. These vesicles cross the cell wall to reach the extracellular space, where the polysaccharide is supposedly used for capsule growth or delivered into host tissues. Galectin-3, a glycan-binding protein, controls a broad spectrum of immunological functions, including cell adhesion, migration, activation, apoptosis and cytokine secretion within innate and adaptive immune compartments. During postdoctoral stage in the Albert Einstein College of Medicine of New York, we have verified that galectin-3 has lytic effect on extracellular vesicles of C. neoformans and we also have detected elevated levels of galectin-3 in the course of cryptococcosis murine experimental. These observations motivate the proposal to study the galectin-3 interference in the course of infection by C. neoformans, furthermore to better understand the effect of galectin-3 on the extracellular vesicles produced by C. neoformans. More specifically, we propose to do the following aims: (1) to dissect the responsible interactions by lytic effect of galectin-3 on the extracelular vesicles from C. neoformans, (2) to investigate the regulated expression and distribution of galectin-3 in murine tissues infected by C. neoformans, (3) to compare the survival of C. neoformans infection between wild and knockout (galectin-3 KO) mice, (4) to investigate the role of galectin-3 in the course of C. neoformans infection in mice through histopathological and immunological parameters, (5) to analyze comparatively the genic profile of the host innate immune response, during cryptococcosis by PCR Array, according to genic modulation to antifungal response, as well as in addition to compare with the genic modulation of the galectin-3 KO mice infected by C. neoformans. Our expectation is that galectin-3 may be responsible for important functions on the infection process by C. neoformans. (AU)

Articles published in Agência FAPESP about the research grant
Protein that inhibits fungal infection is identified  

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANNICK RIES, LAURE NICOLAS; STEENWYK, JACOB L.; DE CASTRO, PATRICIA ALVES; ALMEIDA DE LIMA, POLLYNE BORBOREMA; ALMEIDA, FAUSTO; DE ASSIS, LEANDRO JOSE; MANFIOLLI, ADRIANA OLIVEIRA; TAKAHASHI-NAKAGUCHI, AZUSA; KUSUYA, YOKO; HAGIWARA, DAISUKE; TAKAHASHI, HIROKI; WANG, XI; OBAR, JOSHUA J.; ROKAS, ANTONIS; GOLDMAN, GUSTAVO H. Nutritional Heterogeneity Among Aspergillus fumigatus Strains Has Consequences for Virulence in a Strain- and Host-Dependent Manner. FRONTIERS IN MICROBIOLOGY, v. 10, APR 24 2019. Web of Science Citations: 0.
HATANAKA, OTAVIO; REZENDE, CAROLINE PATINI; MORENO, PEDRO; FERNANDES, FABRICIO FREITAS; MARTINS OLIVEIRA BRITO, PATRICIA KELLEN; MARTINEZ, ROBERTO; COELHO, CAROLINA; ROQUE-BARREIRA, MARIA CRISTINA; CASADEVALL, ARTURO; ALMEIDA, FAUSTO. Galectin-3 Inhibits Paracoccidioides brasiliensis Growth and Impacts Paracoccidioidomycosis through Multiple Mechanisms. MSPHERE, v. 4, n. 2 MAR-APR 2019. Web of Science Citations: 0.
ALMEIDA, FAUSTO; RODRIGUES, MARCIO L.; COELHO, CAROLINA. The Still Underestimated Problem of Fungal Diseases Worldwide. FRONTIERS IN MICROBIOLOGY, v. 10, FEB 12 2019. Web of Science Citations: 1.
BITENCOURT, TAMIRES APARECIDA; REZENDE, CAROLINE PATINI; QUARESEMIN, NATALIA RENAULT; MORENO, PEDRO; HATANAKA, OTAVIO; ROSSI, ANTONIO; MARTINEZ-ROSSI, NILCE MARIA; ALMEIDA, FAUSTO. Extracellular Vesicles From the Dermatophyte Trichophyton interdigitale Modulate Macrophage and Keratinocyte Functions. FRONTIERS IN IMMUNOLOGY, v. 9, OCT 9 2018. Web of Science Citations: 4.
ROCHA, MARINA CAMPOS; DE GODOY, KRISSIA FRANCO; BANNITZ-FERNANDES, RENATA; MARILHANO FABRI, JOAO H. T.; FERRARI BARBOSA, MAYRA M.; DE CASTRO, PATRICIA ALVES; ALMEIDA, FAUSTO; GOLDMAN, GUSTAVO HENRIQUE; DA CUNHA, ANDERSON FERREIRA; NETTO, LUIS E. S.; DE OLIVEIRA, MARCOS ANTONIO; MALAVAZI, IRAN. Analyses of the three 1-Cys Peroxiredoxins from Aspergillus fumigatus reveal that cytosolic Prx1 is central to H2O2 metabolism and virulence. SCIENTIFIC REPORTS, v. 8, AUG 17 2018. Web of Science Citations: 4.
ALMEIDA, FAUSTO; WOLF, JULIE M.; DA SILVA, THIAGO APARECIDO; DELEON-RODRIGUEZ, CARLOS M.; REZENDE, CAROLINE PATINI; PESSONI, ANDRE MOREIRA; FERNANDES, FABRICIO FREITAS; SILVA-ROCHA, RAFAEL; MARTINEZ, ROBERTO; RODRIGUES, MARCIO L.; ROQUE-BARREIRA, MARIA CRISTINA; CASADEVALL, ARTURO. Galectin-3 impacts Cryptococcus neoformans infection through direct antifungal effects. NATURE COMMUNICATIONS, v. 8, DEC 6 2017. Web of Science Citations: 13.
DA SILVA, THIAGO APARECIDO; ROQUE-BARREIRA, MARIA CRISTINA; CASADEVALL, ARTURO; ALMEIDA, FAUSTO. Extracellular vesicles from Paracoccidioides brasiliensis induced M1 polarization in vitro. SCIENTIFIC REPORTS, v. 6, OCT 24 2016. Web of Science Citations: 13.

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