A Mixture of Polyunsaturated Fatty Acids omega-3 and omega-6 Reduces Melanoma Growth by Inhibiting Inflammatory Mediators in the Murine Tumor Microenvironment

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Almeida, Ewin B. ^[1] ; Silva, Karina P. H. ^[1] ; Paixao, Vitoria ^[1] ; do Amaral, Jonatas B. ^[1] ; Rossi, Marcelo ^[1] ; Xavier-Navarro, Roberta A. ^[2] ; Barros, Karina V. ^[3] ; Silveira, Vera L. F. ^[4] ; Vieira, Rodolfo P. ^{[5, 6, 7, 8]} ; Oliveira, Luis V. F. ^[9] ; Perez, Elizabeth C. ^[10] ; Jasiulionis, Miriam G. ^[11] ; Bachi, Andre L. L. ^{[1, 5]}

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^[1] Fed Univ Sao Paulo UNIFESP, Dept Otorhinolaryngol Head & Neck Surg, ENT Res Lab, BR-04039002 Sao Paulo - Brazil

^[2] Samaritan Hosp Blood Bank, BR-01232910 Sao Paulo - Brazil

^[3] Danone Nutr, BR-01311000 Sao Paulo - Brazil

^[4] Fed Univ Sao Paulo UNIFESP, Dept Biol Sci, BR-09972270 Sao Paulo - Brazil

^[5] Brazilian Inst Teaching & Res Pulm & Exercise Imm, BR-12245520 Sao Paulo - Brazil

^[6] Univ Brasil, Postgrad Program Bioengn, BR-08230030 Sao Paulo - Brazil

^[7] Fed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, BR-11015020 Sao Paulo - Brazil

^[8] Anhembi Morumbi Univ, Sch Med, BR-12230002 Sao Paulo - Brazil

^[9] Univ Ctr Anapolis UniEvangel, BR-75083515 Anapolis - Brazil

^[10] Paulista Univ UNIP, Environm & Expt Pathol, BR-04057000 Sao Paulo - Brazil

^[11] Fed Univ Sao Paulo UNIFESP, Pharmacol Dept, Lab Ontogeny & Epigenet, BR-04044020 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 15 AUG 2019.
• Citações Web of Science: 0

Resumo

Background: Although it has been previously demonstrated that acute inflammation can promote the tumor growth of a sub-tumorigenic dose of melanoma cells through of 5-lipoxygenase inflammatory pathway and its product leukotriene B-4,B- and also that the peritumoral treatment with eicosapentaenoic acid and its product, leukotriene B-5, reduces the tumor development, the effect of the treatment by gavage with omega-3 and omega-6 in the tumor microenvironment favorable to melanoma growth associated with acute inflammation has never been studied. Methods: C57BL/6 mice were coinjected with 1 x 10(6) apoptotic cells plus 1 x 10(3) viable melanoma cells into the subcutaneous tissue and treated by gavage with omega-3-rich fish oil or omega-6-rich soybean oil or a mixture of these oils (1:1 ratio) during five consecutive days. Results: The treatment by gavage with a mixture of fish and soybean oils (1:1 ratio) both reduced the melanoma growth and the levels of leukotriene B-4 (LTB4), prostaglandin E-2 (PGE(2)), PGE(2)/prostaglandin E-3 (PGE(3)) ratio, and CXC ligand 1 (CXCL1) and increased the levels of interleukin 10 (IL-10) to IL-10/CXCL1 ratio in the melanoma microenvironment. Conclusion: The oral administration of a 1:1 mixture of fish oil and soybean oil was able to alter the release of inflammatory mediators that are essential for a microenvironment favorable to the melanoma growth in mice, whereas fish oil or soybean oil alone was ineffective. (AU)

• Processo FAPESP: 12/15165-2 - Papel da sinalização purinérgica e da sinalização SOCS-JAK-STAT nos efeitos antiinflamatórios do treinamento aeróbio em modelos experimentais de asma e em indivíduos asmáticos
• Beneficiário: Rodolfo de Paula Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 08/50632-5 - Identificacao e caracterizacao de molecula expressa nos linfocitos b-1 que induz aumento o potencial metastatico de celulas de melanoma murino b16.
• Beneficiário: ELIZABETH CRISTINA PEREZ HURTADO
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/04845-3 - INFLUÊNCIA DA INGESTÃO DIÁRIA DO Lactobacillus casei Shirota (LcS) NA RESPOSTA IMUNE SISTÊMICA E DAS VIAS AÉREAS SUPERIORES DE MARATONISTAS AMADORES QUE APRESENTEM MOLÉSTIAS DE VIAS AÉREAS SUPERIORES
• Beneficiário: André Luis Lacerda Bachi
• Modalidade de apoio: Auxílio à Pesquisa - Regular