Endogenous protection against the 6-OHDA model of Parkinson's disease in the Amazonian rodent Proechimys

Autor(es)

Guimaraes Marques, Marcia Jonathas ^[1] ; Real, Caroline Cristiano ^{[2, 3]} ; Victorino, Daniella Balduino ^[1] ; Britto, Luiz Roberto ^[2] ; Cavalheiro, Esper Abrao ^[1] ; Scorza, Fulvio Alexandre ^[1] ; Ferraz, Henrique Ballalai ^[4] ; Scorza, Carla Alessandra ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Paulista Med Sch, Discipline Neurosci, Dept Neurol & Neurosurg, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Lab Cellular Neurobiol, Dept Biophys & Physiol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Lab Nucl Med LIM 43, Dept Radiol & Oncol, Fac Med, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Movement Disorders Unit, Dept Neurol & Neurosurg, Paulista Med Sch, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neuroscience Letters; v. 709, SEP 14 2019.
• Citações Web of Science: 1

Resumo

Background Proechimys, an epilepsy-resistant rodent from Amazon Rainforest, is a promising alternative animal model for studying neurodegenerative disorders. Objectives: To evaluate behavioral and immunohistological changes in Proechimys after 6 - OHDA-induced model of PD. Methods: Following unilateral injections of 6 - OHDA into striatum, animals were assessed for exploratory behavior using the cylinder test. Brain sections were submitted to immunohistochemistry for tyrosine hydroxylase (TH), glial fibrillary acidic protein (GFAP), and ionized calcium-binding adaptor molecule 1 (lba-1). Results: We observed normal exploratory behavior during cylinder test in all animals. We could not detect changes in the expression of TH in both striatum and SNc, suggesting that Proechimys is resistant to dopaminergic neuronal degeneration. Glial activation was observed by an increase in Iba-1 expression in both striatum and SNc, and by an increase in GFAP expression in striatum. Conclusions: Proechimys represents a promising animal model for studying the mechanisms underlying the susceptibility of dopaminergic neurons to degeneration induced by 6 - OHDA. (AU)

• Processo FAPESP: 16/17746-3 - Avaliação dos efeitos de benzodiazepínicos sobre a atividade eletrofisiológica cerebral in vivo e a viabilidade neuronal in vitro em camundongos da linhagem Ts65Dn
• Beneficiário: Daniella Balduino Victorino
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/25049-2 - Correlação de parâmetros celulares, moleculares, comportamentais e neurofuncionais na Doença de Parkinson com o exercício físico em modelo animal e em tecido humano postmortem
• Beneficiário: Caroline Cristiano Real Gregório
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/06879-2 - Dinâmica cerebral após insulto isquêmico em um roedor da Floresta Amazônica
• Beneficiário: Carla Alessandra Scorza Bahi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/50329-6 - Neurociência translacional da doença de Alzheimer: estudos pré-clínicos e clínicos do peptídeo b-amiloide e outros biomarcadores
• Beneficiário: Geraldo Busatto Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático