Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice

Autor(es)

Mendes de Farias, Talita da Silva ^[1] ; Cruz, Maysa Mariana ^[1] ; da Cunha de Sa, Roberta Cavalcante ^[1] ; Severi, Ilenia ^{[2, 3]} ; Perugini, Jessica ^{[2, 3]} ; Senzacqua, Martina ^{[2, 3]} ; Cerutti, Suzete Maria ^{[1, 4]} ; Giordano, Antonio ^{[2, 3]} ; Cinti, Saverio ^{[2, 3]} ; Cardoso Alonso-Vale, Maria Isabel ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Inst Environm Sci Chem & Pharmaceut, Postgrad Program Chem Biol, Diadema - Brazil

^[2] Univ Ancona, Dept Expt & Clin Med, Politecn Marche, Ancona - Italy

^[3] Univ Ancona, Ctr Obes, Politecn Marche, Ancona - Italy

^[4] Univ Fed Sao Paulo UNIFESP, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN ENDOCRINOLOGY; v. 10, NOV 5 2019.
• Citações Web of Science: 0

Resumo

Obesity results from critical periods of positive energy balance characterized by caloric intake greater than energy expenditure. This disbalance promotes adipose tissue dysfunction which is related to other comorbidities. Melatonin is a low-cost therapeutic agent and studies indicate that its use may improve obesity-related disorders. To evaluate if the melatonin is efficient in delaying or even blocking the damages caused by excessive ingestion of a high-fat diet (HFD) in mice, as well as improving the inflammatory profile triggered by obesity herein, male C57BL/6 mice of 8 weeks were induced to obesity by a HFD and treated for 10 weeks with melatonin. The results demonstrate that melatonin supplementation attenuated serum triglyceride levels and total and LDL cholesterol and prevented body mass gain through a decreased lipogenesis rate and increased lipolytic capacity in white adipocytes, with a concomitant increment in oxygen consumption and Pgc1a and Prdm16 expression. Altogether, these effects prevented adipocyte hypertrophy caused by HFD and reflected in decreased adiposity. Finally, melatonin supplementation reduced the crown-like-structure (CLS) formation, characteristic of the inflammatory process by macrophage infiltration into white adipose tissue of obese subjects, as well as decreased the gene expression of inflammation-related factors, such as leptin and MCP1. Thus, the melatonin can be considered a potential therapeutic agent to attenuate the metabolic and inflammatory disorders triggered by obesity. (AU)

• Processo FAPESP: 18/05485-6 - Efeitos da dieta hiperlipídica e da suplementação com ácidos graxos ômega-7 e ômega-3 sobre o processo de browning, proliferação e diferenciação de células mesenquimais contidas na fração celular estromal do tecido adiposo
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/08582-0 - Efeitos do tratamento com óleo de peixe sobre as disfunções metabólicas e endócrinas de adipócitos isolados de animais com síndrome metabólica
• Beneficiário: Roberta Dourado Cavalcante da Cunha de Sá
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/07638-9 - Efeito da suplementação com melatonina no processo de transdiferenciação do tecido adiposo branco em tecido adiposo marrom em camundongos obesos
• Beneficiário: Talita da Silva Mendes de Farias Carvalho
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 15/03554-2 - Os efeitos da suplementação com melatonina no quadro do desenvolvimento da obesidade em camundongos
• Beneficiário: Talita da Silva Mendes de Farias Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado