Circulating plasma microRNAs dysregulation and metabolic endotoxemia induced by a high-fat high-saturated diet

Autor(es)

Quintanilha, Bruna Jardim ^{[1, 2]} ; Pinto Ferreira, Ludmila Rodrigues ^[3] ; Ferreira, Frederico Moraes ^[4] ; Neto, Edecio Cunha ^[4] ; Sampaio, Geni Rodrigues ^[2] ; Rogero, Marcelo Macedo ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo Res Fdn, Res Innovat & Disseminat Ctr, CEPID FAPESP, Food Res Ctr FoRC, BR-05468140 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, Nutr Genom & Inflammat Lab, BR-01296904 Sao Paulo - Brazil

^[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Morfol, BR-31270910 Belo Horizonte, MG - Brazil

^[4] Univ Sao Paulo, Sch Med, Heart Inst, Lab Immunol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical Nutrition; v. 39, n. 2, p. 554-562, FEB 2020.
• Citações Web of Science: 2

Resumo

High-fat diet increase two to three times the plasma lipopolysaccharide (LPS) levels and induce subclinical inflammation. Diet can modify gene expression due to epigenetic processes related to MicroRNAs (miRNAs). MicroRNAs (miRNAs) play important role in the post-transcriptional mechanisms involved in regulation of expression of genes related to the inflammatory response. Also, diet can indirectly induce post-transcriptional regulation of gene expression by miRNAs, which may affect the risk for the development of chronic diseases. Objective: This study investigated the effect of high-fat high-saturated meal ingestion on plasma miRNA expression and LPS levels during the postprandial period in healthy women. Methods: An interventional study was carried out in which a high-fat breakfast (1067.45 kcal), composed mainly of saturated fatty acids (56 g), and 500 mL of water, was offered. Blood samples were collected at baseline and 1, 3 and 5 h after meal intake. The studied population consisted of healthy women (n = 11), aged between 20 and 40 years, and body mass index (BMI) between 18.5 and 25 kg/m(2). Plasma levels of lipid profile, cytokines, adhesion molecules, and LPS were measured at the 3 time points. A profile of 752 human plasma miRNA expression was analyzed by real-time PCR assay. These analyzes were performed for all blood collection time-points. Results: Expression profile analysis revealed 33 differentially expressed plasma circulating miRNAs compared to that of the control group. MiR-145-5p and miR-200 were differentially modulated in all time-points post meal consumption. In addition, there was a significant increase in plasma LPS, triglycerides, myristic and palmitic saturated fatty acids levels at the 3 time-points in comparison with the control basal levels. We also observed increased levels of the plasma tumor necrosis factor alpha (TNF-alpha) cytokine and the vascular cell adhesion molecule 1 (VCAM-1) levels after 5 h post meal ingestion. Conclusion: Ingestion of high-fat high-saturated meal was able to induce metabolic endotoxemia and increase the expression of pro-inflammatory molecules such as TNF-alpha and VCAM-1, as well as modulating circulating miRNAs possibly controlling inflammatory and lipid metabolism proteins at the postprandial period. (C) 2019 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. (AU)

• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs