Insoluble-Bound Polyphenols Released from Guarana Powder: Inhibition of Alpha-Glucosidase and Proanthocyanidin Profile

Autor(es)

da Costa Pinaffi, Ana Clara ^[1] ; Sampaio, Geni Rodrigues ^[1] ; Soares, Maiara Jurema ^[1] ; Shahidi, Fereidoon ^[2] ; de Camargo, Adriano Costa ^[3] ; Torres, Elizabeth A. F. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Publ Hlth, 715 Dr Arnaldo Ave, BR-01246904 Sao Paulo, SP - Brazil

^[2] Mem Univ Newfoundland, Dept Biochem, St John, NF A1B 3X9 - Canada

^[3] Pontificia Univ, Fac Agron & Ingn Forestal, Dept Ciencias Vegetales, Casilla 306-22, Santiago - Chile

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 25, n. 3 FEB 1 2020.
• Citações Web of Science: 6

Resumo

The Brazilian Food Supplement Law recently recognized that guarana (Paullinia cupana) contains bioactive substances, hence supporting its role as a functional food ingredient. The health benefits of guarana are associated, at least in part, to its phenolic compounds. However, to the best of our knowledge, there is no literature addressing the presence of phenolic compounds in the fraction containing insoluble-bound compounds and its contribution in terms of alpha-glucosidase inhibition. The concentration of phenolic extracts released from the insoluble-bound fraction required to inhibit 50% of alpha-glucosidase (IC50) activity was 5.8-fold lower than that present in the soluble counterpart. Both fractions exhibited a mixed inhibition mode. Fourteen proanthocyanidins (dimers to tetramers) present in the insoluble-bound fraction were tentatively identified by MALDi-TOF-MS. Future studies aiming at increasing the concentration of the soluble counterpart are deemed necessary. The results presented here enhance the phenolic database of guarana and have a practical impact on the procurement of nutraceuticals and functional ingredients related to the prevention and/or management of type 2 diabetes. The Brazilian normative on food supplements has been recently revised. This study lends support to the future inclusion of guarana powder in the list of sources of proanthocyanidins for the industry of food supplements. (AU)

• Processo FAPESP: 17/20039-0 - Efeitos do guaraná (Paullinia cupana) no metabolismo lipídico e bioacessibilidade de seus polifenóis em genes relacionados à absorção de colesterol em células Caco-2
• Beneficiário: Elizabeth Aparecida Ferraz da Silva Torres
• Modalidade de apoio: Auxílio à Pesquisa - Regular