Using Cytometry for Investigation of Purinergic Signaling in Tumor-Associated Macrophages

Autor(es)

Arnaud-Sampaio, Vanessa F. ^[1] ; Rabelo, Izadora L. A. ^[1] ; Bento, Carolina A. ^[1] ; Glaser, Talita ^[1] ; Bezerra, Jean ^[1] ; Coutinho-Silva, Robson ^[2] ; Ulrich, Henning ^[1] ; Lameu, Claudiana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem, Dept Biochem, Ave Prof Lineu Prestes 748, Sala 966, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Biophys Inst Carlos Chagas Filho, Immunophysiol Lab, Rio De Janeiro - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Cytometry Part A; v. 97, n. 11 JUL 2020.
• Citações Web of Science: 1

Resumo

Tumor-associated macrophages are widely recognized for their importance in guiding pro-tumoral or antitumoral responses. Mediating inflammation or immunosuppression, these cells support many key events in cancer progression: cell growth, chemotaxis, invasiveness, angiogenesis and cell death. The communication between cells in the tumor microenvironment strongly relies on the secretion and recognition of several molecules, including damage-associated molecular patterns (DAMPs), such as adenosine triphosphate (ATP). Extracellular ATP (eATP) and its degradation products act as signaling molecules and have extensively described roles in immune response and inflammation, as well as in cancer biology. These multiple functions highlight the purinergic system as a promising target to investigate the interplay between macrophages and cancer cells. Here, we reviewed purinergic signaling pathways connecting cancer cells and macrophages, a yet poorly investigated field. Finally, we present a new tool for the characterization of macrophage phenotype within the tumor. Image cytometry emerges as a cutting-edge tool, capable of providing a broad set of information on cell morphology, expression of specific markers, and its cellular or subcellular localization, preserving cell-cell interactions within the tumor section and providing high statistical strength in small-sized experiments. Thus, image cytometry allows deeper investigation of tumor heterogeneity and interactions between these cells. (c) 2020 International Society for Advancement of Cytometry (AU)

• Processo FAPESP: 17/06132-7 - Estudo das isoformas do receptor P2X7 na manutenção de células-tronco cancerígenas e metástase
• Beneficiário: Izadora Lorrany Alves Rabelo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/13345-1 - Doença de Huntington: envolvimento de Huntingtin no controle do comprometimento celular
• Beneficiário: Talita Glaser
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/07366-4 - Receptores de purinas e cininas como alvos de estudo e intervenção terapêutica em doenças neurológicas
• Beneficiário: Alexander Henning Ulrich
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/14993-5 - Influência dos fenótipos M1 e M2 de macrófagos e do receptor P2X7 na metástase de neuroblastoma
• Beneficiário: Carolina Adriane Bento
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/19128-2 - Mecanismos de metástase de tumores infantis para a medula óssea
• Beneficiário: Claudiana Lameu
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores