Lipid excess affects chaperone-mediated autophagy in hypothalamus

Autor(es):Mostrar menos -

Portovedo, M. ^{[1, 2]} ; Reginato, A. ^[1] ; Miyamoto, J. E. ^[1] ; Simino, L. A. ^[1] ; Hakim, M. P. ^[1] ; Campana, M. ^[3] ; Leal, R. F. ^[4] ; Ignacio-Souza, L. M. ^[1] ; Torsoni, M. A. ^[1] ; Magnan, C. ^[3] ; Le Stunff, H. ^{[3, 5]} ; Torsoni, A. S. ^[1] ; Milanski, M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Lab Metab Disorders, Fac Appl Sci, BR-13484350 Limeira - Brazil

^[2] Univ Estadual Campinas, Lab Immunoinflammat, Inst Biol, BR-13083862 Campinas - Brazil

^[3] Univ Paris, CNRS, BFA, UMR 8251, F-75013 Paris - France

^[4] Univ Estadual Campinas, Dept Surg, Colorectal Surg Unit, Sch Med Sci, IBD Res Lab, BR-13083878 Campinas - Brazil

^[5] Univ Paris Saclay, Paris Saclay Inst Neurosci, Univ Paris Sud, CNRS UMR 9197, Orsay - France

• Tipo de documento: Artigo Científico
• Fonte: Biochimie; v. 176, p. 110-116, SEP 2020.
• Citações Web of Science: 1

Resumo

Obesity is a major health problem worldwide. Overweight and obesity directly affect health-related quality of life and also have an important economic impact on healthcare systems. In experimental models, obesity leads to hypothalamic inflammation and loss of metabolic homeostasis. It is known that macroautophagy is decreased in the hypothalamus of obese mice but the role of chaperone-mediated autophagy is still unknown. In this study, we aimed to investigate the role of hypothalamic chaperone-mediated autophagy in response to high-fat diet and also the direct effect of palmitate on hypothalamic neurons. Mice received chow or high-fat diet for 3 days or 1 week. At the end of the experimental protocol, chaperone-mediated autophagy in hypothalamus was investigated, as well as cytokines expression. In other set of experiments, neuronal cell lines were treated with palmitic acid, a saturated fatty acid. We show that chaperone-mediated autophagy is differently regulated in response to high-fat diet intake for 3 days or 1 week. Also, when hypothalamic neurons are directly exposed to palmitate there is activation of chaperone-mediated autophagy. High-fat diet causes hypothalamic inflammation concomitantly to changes in the content of chaperone-mediated autophagy machinery. It remains to be studied the direct role of inflammation and lipids itself on the activation of chaperone-mediated autophagy in the hypothalamus in vivo and also the neuronal implications of chaperone-mediated autophagy inhibition in response to obesity. (c) 2020 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. (AU)

• Processo FAPESP: 13/10911-0 - Modulação da autofagia mediada por chaperonas em hipotálamo de animais com obesidade induzida por dieta hiperlipídica.
• Beneficiário: Mariana Portovedo de Oliveira Araújo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/11751-0 - Efeito de ácidos graxos saturados sob mecanismos moleculares envolvidos no controle central da fome: imunomodulação e autofagia
• Beneficiário: Adriana Souza Torsoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular