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Venom complexity of Bothrops atrox (common lancehead) siblings

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Autor(es):
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Hatakeyama, Daniela Miki [1, 2, 3] ; Tasima, Lidia Jorge [1, 2, 3] ; Bravo-Tobar, Cesar Adolfo [1, 2, 3] ; Serino-Silva, Caroline [1, 2, 3] ; Tashima, Alexandre Keiji [4] ; Bittencourt Rodrigues, Caroline Fabri [1, 2, 3] ; Aguiar, Weslei da Silva [1, 2, 3] ; Galizio, Nathalia da Costa [1, 2, 3] ; Venancio de Lima, Eduardo Oliveira [1] ; Kavazoi, Victor Koiti [1, 2, 3] ; Gutierrez-Marin, Juan David [1, 2, 3] ; de Farias, Iasmim Baptista [1, 2, 3] ; Sant'Anna, Savio Stefanini [1] ; Grego, Kathleen Fernandes [1] ; de Morais-Zani, Karen [1, 2, 3] ; Tanaka-Azevedo, Anita Mitico [1, 2, 3]
Número total de Autores: 16
Afiliação do(s) autor(es):
[1] Butantan Inst, Lab Herpetol, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Interinst Grad Program Biotechnol IPT, IBU, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, USP, Sao Paulo, SP - Brazil
[4] Fed Univ Sao Paulo UNIFESP, Dept Biochem, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 26, OCT 12 2020.
Citações Web of Science: 0
Resumo

Background: Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of Bothrops atrox snake venom using siblings is poorly investigated. Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Differences in the venom composition of Bothrops genus have been linked to several factors such as ontogeny, geographical distribution, prey preferences and sex. Thus, in the current study, venom samples of Bothrops atrox male and female siblings were analyzed in order to compare their biochemical and biological characteristics. Methods: Venoms were collected from five females and four males born from a snake captured from the wild in Sao Bento (Maranhao, Brazil), and kept in the Laboratory of Herpetology of Butantan Intitute. The venoms were analyzed individually and as a pool of each gender. The assays consisted in protein quantification, 1-DE, mass spectrometry, proteolytic, phospholipase A 2, L-amino acid oxidase activities, minimum coagulant dose upon plasma, minimum hemorrhagic dose and lethal dose 50%. Results: Electrophoretic profiles of male's and female's venom pools were quite similar, with minor sex-based variation. Male venom showed higher LAAO, PLA(2) and hemorrhagic activities, while female venom showed higher coagulant activity. On the other hand, the proteolytic activities did not show statistical differences between pools, although some individual variations were observed. Meanwhile, proteomic profile revealed 112 different protein compounds; of which 105 were common proteins of female's and male's venom pools and seven were unique to females. Despite individual variations, lethality of both pools showed similar values. Conclusion: Although differences between female and male venoms were observed, our results show that individual variations are significant even between siblings, highlighting that biological activities of venoms and its composition are influenced by other factors beyond gender. (AU)

Processo FAPESP: 17/16908-2 - Caracterização proteômica e das atividades enzimáticas e fisiopatológicas do veneno das espécies de serpentes que compõem o grupo Bothrops neuwiedi
Beneficiário:Karen de Morais Zani
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/25786-0 - Estudo dos inibidores de fosfolipase A2 (PLIs) presentes nos plasmas das serpentes peçonhentas e não-peçonhentas e avaliação da atividade neutralizante destes sobre as atividades da fosfolipase A2 (PLA2) dos venenos de serpentes
Beneficiário:Anita Mitico Tanaka-Azevedo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/01890-0 - Estudo comparativo da composição do veneno das serpentes Crotalus durissus terrificus nascidas em cativeiro no Laboratório de Herpetologia do Instituto Butantan e do veneno crotálico de referência nacional
Beneficiário:Anita Mitico Tanaka-Azevedo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/20106-9 - Peptidômica dos venenos de serpentes e aranhas brasileiras
Beneficiário:Alexandre Keiji Tashima
Linha de fomento: Auxílio à Pesquisa - Regular