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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Venom complexity of Bothrops atrox (common lancehead) siblings

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Author(s):
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Hatakeyama, Daniela Miki [1, 2, 3] ; Tasima, Lidia Jorge [1, 2, 3] ; Bravo-Tobar, Cesar Adolfo [1, 2, 3] ; Serino-Silva, Caroline [1, 2, 3] ; Tashima, Alexandre Keiji [4] ; Bittencourt Rodrigues, Caroline Fabri [1, 2, 3] ; Aguiar, Weslei da Silva [1, 2, 3] ; Galizio, Nathalia da Costa [1, 2, 3] ; Venancio de Lima, Eduardo Oliveira [1] ; Kavazoi, Victor Koiti [1, 2, 3] ; Gutierrez-Marin, Juan David [1, 2, 3] ; de Farias, Iasmim Baptista [1, 2, 3] ; Sant'Anna, Savio Stefanini [1] ; Grego, Kathleen Fernandes [1] ; de Morais-Zani, Karen [1, 2, 3] ; Tanaka-Azevedo, Anita Mitico [1, 2, 3]
Total Authors: 16
Affiliation:
[1] Butantan Inst, Lab Herpetol, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Interinst Grad Program Biotechnol IPT, IBU, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, USP, Sao Paulo, SP - Brazil
[4] Fed Univ Sao Paulo UNIFESP, Dept Biochem, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 26, OCT 12 2020.
Web of Science Citations: 0
Abstract

Background: Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of Bothrops atrox snake venom using siblings is poorly investigated. Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Differences in the venom composition of Bothrops genus have been linked to several factors such as ontogeny, geographical distribution, prey preferences and sex. Thus, in the current study, venom samples of Bothrops atrox male and female siblings were analyzed in order to compare their biochemical and biological characteristics. Methods: Venoms were collected from five females and four males born from a snake captured from the wild in Sao Bento (Maranhao, Brazil), and kept in the Laboratory of Herpetology of Butantan Intitute. The venoms were analyzed individually and as a pool of each gender. The assays consisted in protein quantification, 1-DE, mass spectrometry, proteolytic, phospholipase A 2, L-amino acid oxidase activities, minimum coagulant dose upon plasma, minimum hemorrhagic dose and lethal dose 50%. Results: Electrophoretic profiles of male's and female's venom pools were quite similar, with minor sex-based variation. Male venom showed higher LAAO, PLA(2) and hemorrhagic activities, while female venom showed higher coagulant activity. On the other hand, the proteolytic activities did not show statistical differences between pools, although some individual variations were observed. Meanwhile, proteomic profile revealed 112 different protein compounds; of which 105 were common proteins of female's and male's venom pools and seven were unique to females. Despite individual variations, lethality of both pools showed similar values. Conclusion: Although differences between female and male venoms were observed, our results show that individual variations are significant even between siblings, highlighting that biological activities of venoms and its composition are influenced by other factors beyond gender. (AU)

FAPESP's process: 17/16908-2 - Proteomic characterization and enzymatic and pathophysiological activities of the venom of the snake species that compose the Bothrops neuwiedi group
Grantee:Karen de Morais Zani
Support Opportunities: Regular Research Grants
FAPESP's process: 18/25786-0 - Phospholipase A2 inhibitors (PLIs) present in venomous and non-venomous snake plasmas and evaluation of the neutralizing activity of these inhibitors on the phospholipase A2 (PLA2) activities of snake venoms
Grantee:Anita Mitico Tanaka-Azevedo
Support Opportunities: Regular Research Grants
FAPESP's process: 17/01890-0 - Comparative study of Crotalus durissus terrificus snake venoms born in captivity in the Laboratory of Herpetology of Butantan Institute and the national crotalic reference venom
Grantee:Anita Mitico Tanaka-Azevedo
Support Opportunities: Regular Research Grants
FAPESP's process: 17/20106-9 - Peptidomics of Brazilian snake and spider venoms
Grantee:Alexandre Keiji Tashima
Support Opportunities: Regular Research Grants