Rosettes integrity protects Plasmodium vivax of being phagocytized

Autor(es):Mostrar menos -

Albrecht, Letusa ^{[1, 2]} ; Lopes, Stefanie C. P. ^{[3, 4]} ; Iung Enembreck da Silva, Ana Beatriz ^[1] ; Barbosa, Vanessa ^{[3, 4]} ; Almeida, Rodrigo P. ^[1] ; Siqueira, Andre M. ^[5] ; Leite, Juliana Almeida ^[2] ; Bittencourt, Najara C. ^[2] ; dos Santos, Hellen Geremias ^[1] ; Bourgard, Catarina ^[2] ; Cardoso Garcia, Luiz Fernando ^[1] ; Kayano, Ana Carolina A. V. ^{[1, 2]} ; Soares, Irene S. ^[6] ; Russell, Bruce ^[7] ; Renia, Laurent ^[8] ; Lacerda, Marcus V. G. ^{[3, 4]} ; Costa, Fabio T. M. ^[2]

Afiliação do(s) autor(es)

^[1] Fiocruz Parana, Inst Carlos Chagas, Lab Pesquisa Apicomplexa, Curitiba, Parana - Brazil

^[2] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Genet Evolucao Microbiol & Imunol, Lab Doencas Tropicais Prof Luiz Jacintho da Silva, Campinas, SP - Brazil

^[3] Fundacao Med Trop Dr Heitor Vieira Dourado, Gerencia Malaria, Manaus, Amazonas - Brazil

^[4] Fiocruz Amazonia, Inst Leonidas & Maria Deane, Manaus, Amazonas - Brazil

^[5] Fiocruz MS, Inst Nacl Infectol Evandro Chagas, Rio De Janeiro, RJ - Brazil

^[6] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, SP - Brazil

^[7] Univ Otago, Dept Microbiol & Immunol, Dunedin - New Zealand

^[8] Agcy Sci Technol & Res, Singapore Immunol Network, Singapore - Singapore

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 OCT 7 2020.
• Citações Web of Science: 2

Resumo

Plasmodium vivax is the most prevalent cause of malaria outside of Africa. P. vivax biology and pathogenesis are still poorly understood. The role of one highly occurring phenotype in particular where infected reticulocytes cytoadhere to noninfected normocytes, forming rosettes, remains unknown. Here, using a range of ex vivo approaches, we showed that P. vivax rosetting rates were enhanced by plasma of infected patients and that total immunoglobulin M levels correlated with rosetting frequency. Moreover, rosetting rates were also correlated with parasitemia, IL-6 and IL-10 levels in infected patients. Transcriptomic analysis of peripheral leukocytes from P. vivax-infected patients with low or moderated rosetting rates identified differentially expressed genes related to human host phagocytosis pathway. In addition, phagocytosis assay showed that rosetting parasites were less phagocyted. Collectively, these results showed that rosette formation plays a role in host immune response by hampering leukocyte phagocytosis. Thus, these findings suggest that rosetting could be an effective P. vivax immune evasion strategy. (AU)

• Processo FAPESP: 13/25807-4 - Dissecando os mecanismos de infectividade em Plasmodium - modelo experimental de Plasmodium yoelii com implicações para o estudo em Plasmodium vivax
• Beneficiário: Juliana Almeida Leite
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/16525-2 - Plasmodium vivax: patogênese e infectividade
• Beneficiário: Fabio Trindade Maranhão Costa
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/20509-5 - Análise dos mecanismos imunopatológicos e moleculares envolvidos no processo de citoaderência de Plasmodium vivax
• Beneficiário: Catarina Baeta da Luz Bourgard
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/18611-7 - Desenvolvimento de novas ferramentas para busca e validação de alvos moleculares para terapia contra Plasmodium vivax
• Beneficiário: Fabio Trindade Maranhão Costa
• Linha de fomento: Auxílio à Pesquisa - Temático