Hydrophilic interaction liquid chromatography coupled to quadrupole time-of-flight mass spectrometry as a potential combination for the determination of sulfonamide residues in complex infant formula matrices

Autor(es)

Petrarca, Mateus Henrique ^[1] ; de Campos Braga, Patricia Aparecida ^[1] ; Reyes Reyes, Felix Guillermo ^[1] ; Arisseto Bragotto, Adriana Pavesi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, UNICAMP, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chromatography A; v. 1633, DEC 6 2020.
• Citações Web of Science: 0

Resumo

An accurate, sensitive and selective analytical method is proposed for sulfonamide residues analysis in infant formulas based on hydrophilic interaction liquid chromatography (HILIC) and quadrupole time-offlight mass spectrometry in full scan mode. The sample preparation approach involves low-temperature lipid precipitation followed by dispersive solid-phase extraction with PSA and C-18 sorbents, which was successfully optimized using Plackett-Burman design. In order to achieve high analytical sensitivity, the influence of HILIC conditions on sulfonamide ionization was investigated, such as the mobile phase composition, buffer concentration, and sample diluent for injection. The method performance characteristics, including linearity (range 5-120 mu g kg(-1)), reliable limits of quantification (between 5 and 20 mu g kg(-1)), recovery (72.9-109.2%) and precision (coefficient of variation values <= 19.8%) under repeatability and within-laboratory reproducibility conditions, were in accordance with the Codex Alimentarius Commission CAC/GL 71-2009 for quantitative analytical methods for veterinary drug residues in foods. Moreover, adequate identification of the compounds was provided with accurate mass measurement of both pre cursor and fragment ions in one single run. Finally, the developed method was applied to thirty-five powdered milk-based infant formula samples available in the Brazilian market. (c) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/09149-8 - Espectrometria de massas em ciência e tecnologia de alimentos
• Beneficiário: Felix Guillermo Reyes Reyes
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários