Perylenequinones production induced by co-culturing Setophoma sp. and Penicillium brasilianum

Autor(es)

Bazioli, Jaqueline Moraes ^{[1, 2]} ; Fill, Taicia Pacheco ^[2] ; Rocha, Marina Campos ^[3] ; Malavazi, Iran ^[3] ; Rodrigues Filho, Edson ^[4] ; de Medeiros, Livia Soman ^[5]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos, LaBioMMi, Dept Chem, Km 265, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Chem, BR-09913030 Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHYTOCHEMISTRY LETTERS; v. 40, p. 76-83, DEC 2020.
• Citações Web of Science: 1

Resumo

Co-culture strategy can be applied to produce structural diversity of natural products. This approach also enables the understanding of the interaction mechanisms in microbial ecology. The present study aimed at the secondary metabolism diversification of the endophytic fungus Setophoma sp. by co-culturing it with Penicillium brasilianum and Talaromyces wortmannii, species also isolated as endophytes, and with Penicillium digitatum, a phytopathogenic fungus dangerous to Brazilian citriculture. The increase of perylenequinones (stemphyperylenol and derivatives) produced by Setophoma sp. strain was observed in the co-cultures in response to the endophytic P. brasilianum. The induced stemphyperylenol was isolated by combined chromatographic procedures and identified by spectroscopic techniques. Other perylenequinone derivatives were detected by UHPLC-DAD-HRMS based dereplication data analysis. Stemphyperylenol exhibited antifungal effects not only against P. brasilianum, but also against P. digitatum, the main postharvest pathogen of orange fruits, and Aspergillus fumigatus, an important human pathogenic fungus ubiquitous in soil. Preliminary insights concerning the mode of action of stemphyperylenol were determined by evaluating a collection of A. fumigatus mutants. Thus, stemphyperylenol may have the potential for agricultural applications, and it is also promising as a human antifungal compound. Moreover, the co-culture of an endophyte-endophyte system may represent a potential way to achieve the chemical diversity of bioactive molecules. (AU)

• Processo FAPESP: 19/04900-2 - Influência de agentes indutores abióticos no metabolismo secundário de micro-organismos endofíticos
• Beneficiário: Edson Rodrigues Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/00315-5 - A influência de proteínas quinases ativadas por mitógenos (MAPK) na expressão de determinantes genéticos importantes para a virulência de Aspergillus fumigatus
• Beneficiário: Marina Campos Rocha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/06359-7 - Compreensão dos fatores químicos envolvidos no endofitismo x patogenicidade de diferentes isolados de Penicillium brasilianum
• Beneficiário: Taicia Pacheco Fill
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/11563-2 - Prospecção de metabólitos secundários com atividade anticâncer produzidos por fungos fitopatogênicos
• Beneficiário: Jaqueline Moraes Bazioli
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/18584-3 - Prospecção química do metabolismo secundário de fungos endofíticos isolados de maçã (Malus domestica) e goiaba (Psidium guajava)
• Beneficiário: Lívia Soman de Medeiros
• Modalidade de apoio: Bolsas no Brasil - Doutorado