Perylenequinones production induced by co-culturing Setophoma sp. and Penicillium brasilianum

Author(s)

Bazioli, Jaqueline Moraes ^{[1, 2]} ; Fill, Taicia Pacheco ^[2] ; Rocha, Marina Campos ^[3] ; Malavazi, Iran ^[3] ; Rodrigues Filho, Edson ^[4] ; de Medeiros, Livia Soman ^[5]

Affiliation

^[1] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos, LaBioMMi, Dept Chem, Km 265, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Chem, BR-09913030 Diadema, SP - Brazil

• Document type: Journal article
• Source: PHYTOCHEMISTRY LETTERS; v. 40, p. 76-83, DEC 2020.
• Web of Science Citations: 1

Abstract

Co-culture strategy can be applied to produce structural diversity of natural products. This approach also enables the understanding of the interaction mechanisms in microbial ecology. The present study aimed at the secondary metabolism diversification of the endophytic fungus Setophoma sp. by co-culturing it with Penicillium brasilianum and Talaromyces wortmannii, species also isolated as endophytes, and with Penicillium digitatum, a phytopathogenic fungus dangerous to Brazilian citriculture. The increase of perylenequinones (stemphyperylenol and derivatives) produced by Setophoma sp. strain was observed in the co-cultures in response to the endophytic P. brasilianum. The induced stemphyperylenol was isolated by combined chromatographic procedures and identified by spectroscopic techniques. Other perylenequinone derivatives were detected by UHPLC-DAD-HRMS based dereplication data analysis. Stemphyperylenol exhibited antifungal effects not only against P. brasilianum, but also against P. digitatum, the main postharvest pathogen of orange fruits, and Aspergillus fumigatus, an important human pathogenic fungus ubiquitous in soil. Preliminary insights concerning the mode of action of stemphyperylenol were determined by evaluating a collection of A. fumigatus mutants. Thus, stemphyperylenol may have the potential for agricultural applications, and it is also promising as a human antifungal compound. Moreover, the co-culture of an endophyte-endophyte system may represent a potential way to achieve the chemical diversity of bioactive molecules. (AU)

• FAPESP's process: 19/04900-2 - Influence of abiotic inducers agents in secondary metabolism of endophytic microorganisms
• Grantee: Edson Rodrigues Filho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/00315-5 - The influence of mitogen activated protein kinases (MAPK) on the expression of genetic determinants important for Aspergillus fumigatus virulence
• Grantee: Marina Campos Rocha
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/06359-7 - Understanding the chemical factors involved in the endophytism x pathogenicity of different Penicillium brasilianum isolates
• Grantee: Taicia Pacheco Fill
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/11563-2 - Prospecting for anticancer secondary metabolites produced by phytopathogenic fungi
• Grantee: Jaqueline Moraes Bazioli
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 09/18584-3 - Chemical prospecting of the secondary metabolism of endophytic fungi isolated from apple (Malus domestica) and guava fruit (Psidium guajava)
• Grantee: Lívia Soman de Medeiros
• Support Opportunities: Scholarships in Brazil - Doctorate