Alcohol Use Disorder is Associated with Upregulation of MicroRNA-34a and MicroRNA-34c in Hippocampal Postmortem Tissue

Autor(es):Mostrar menos -

Santos-Bezerra, Daniele P. ^[1] ; Cavaleiro, Ana Mercedes ^[1] ; Santos, Aritania Sousa ^[1] ; Suemoto, Claudia Kimie ^[2] ; Pasqualucci, Carlos Augusto ^[3] ; Jacob-Filho, Wilson ^[2] ; Paraizo Leite, Renata Elaine ^{[3, 2]} ; Passarelli, Marisa ^{[4, 5]} ; Nagahashi Marie, Suely Kazue ^[6] ; Machado, Ubiratan Fabres ^[7] ; Correa-Giannella, Maria Lucia ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Carboidratos & Radioimunoensaio LIM 18, Hosp Clin HCFMUSP, Fac Med, Av Dr Arnaldo 455, 3321, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Div Geriatr, Med Sch, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Pathol, Sao Paulo - Brazil

^[4] Univ Nove Julho Uninove, Programa Posgrad Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med, Lab Lipides LIM 10 HCFMUSP, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Dept Neurol, Sch Med, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH; v. 45, n. 1, p. 64-68, JAN 2021.
• Citações Web of Science: 0

Resumo

Background: To investigate epigenetic mechanisms potentially involved in the cognitive decline associated with chronic alcohol intake, we evaluated the expressions of three micro-RNAs (miR-34a, 34b, and -34c) highly expressed in the hippocampus and involved in neuronal physiology and pathology. MiR-34a participates in functioning and survival of mature neurons; miR-34b is associated with Alzheimer-like disorders; and miR-34c is implicated in the memory impairment of Alzheimer disease in rodents and humans. Methods: A total of 69 cases were selected from the Biobank for Aging Studies and categorized according to the absence (n = 50) or presence (n = 19) of alcohol use disorder (AUD). Cases presenting with neuropathological diagnoses of dementias were excluded. Total RNA was extracted from hippocampal paraffinized slices, complementary DNA was synthesized from miRs, and RT-qPCR was performed with TaqMan (R) assays. Results: Higher expressions of miR-34a and miR-34c, but not of miR-34b, were found in the group with AUD in comparison with the group without AUD after adjustment for potential confounders (age, sex, body mass index, presence of hypertension, diabetes mellitus, smoking, and physical inactivity). Conclusions: Hippocampal upregulation of miR-34a and miR-34c may be involved in the cognitive decline associated with chronic alcohol consumption. (AU)

• Processo FAPESP: 18/12265-2 - Alteração na expressão de transportadores de glicose no sistema nervoso central e sua correlação com Diabetes mellitus Tipo 3 (DM3) e Doença de Alzheimer
• Beneficiário: Daniele Pereira dos Santos Bezerra
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/15603-0 - Desvendando mecanismos envolvidos no controle glicêmico e nas complicações crônicas do Diabetes mellitus: contribuições à saúde humana
• Beneficiário: Ubiratan Fabres Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático