Cystatin-like protein of sweet orange (CsinCPI-2) modulates pre-osteoblast differentiation via beta-Catenin involvement

Autor(es)

da Costa Fernandes, Jr., Celio ^[1] ; Rodriguez, Victor Manuel Ochoa ^[2] ; Soares-Costa, Andrea ^[3] ; Cirelli, Joni Augusto ^[4] ; Justino, Daniela Morilha Neo ^[3] ; Roma, Barbara ^[2] ; Zambuzzi, Willian Fernando ^[1] ; Faria, Gisele ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Chem & Biochem, Lab Bioassays & Cell Dynam, Inst Biosci, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Restorat Dent, Araraquara, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Diag & Surg, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 32, n. 4 APR 2021.
• Citações Web of Science: 0

Resumo

Phytocystatins are endogenous cysteine-protease inhibitors present in plants. They are involved in initial germination rates and in plant defense mechanisms against phytopathogens. Recently, a new phytocystatin derived from sweet orange, CsinCPI-2, has been shown to inhibit the enzymatic activity of human cathepsins, presenting anti-inflammatory potential and pro-osteogenic effect in human dental pulp cells. The osteogenic potential of the CsinCPI-2 protein represents a new insight into plants cysteine proteases inhibitors and this effect needs to be better addressed. The aim of this study was to investigate the performance of pre-osteoblasts in response to CsinCPI-2, mainly focusing on cell adhesion, proliferation and differentiation mechanisms. Together our data show that in the first hours of treatment, protein in CsinCPI-2 promotes an increase in the expression of adhesion markers, which decrease after 24 h, leading to the activation of Kinase-dependent cyclines (CDKs) modulating the transition from G1 to S phases cell cycle. In addition, we saw that the increase in ERK may be associated with activation of the differentiation profile, also observed with an increase in the B-Catenin pathway and an increase in the expression of Runx2 in the group that received the treatment with CsinCPI-2. {[}GRAPHICS] . (AU)

• Processo FAPESP: 14/22689-3 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo
• Beneficiário: Willian Fernando Zambuzzi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/24150-5 - Potencial osteo/odontogênico da cistatina Citrus CPI-2, derivada da Citrus sinensis (laranja doce), em células da polpa dental humana
• Beneficiário: Bárbara Roma Mendes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
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• Beneficiário: Andrea Soares da Costa Fuentes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/05784-0 - Efeito da fitocistatina Citrus CPI-2 sobre a proliferação, migração e diferenciação de células da papila apical e polpa dental humanas
• Beneficiário: Gisele Faria
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/21807-6 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo
• Beneficiário: Célio Junior da Costa Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto