Not just a methane source: Amazonian floodplain sediments harbour a high diversity of methanotrophs with different metabolic capabilities

Autor(es)

Gontijo, Julia B. ^[1] ; Paula, Fabiana S. ^{[2, 1]} ; Venturini, Andressa M. ^[1] ; Yoshiura, Caio A. ^[1] ; Borges, Clovis D. ^[1] ; Moura, Jose Mauro S. ^[3] ; Bohannan, Brendan J. M. ^[4] ; Nusslein, Klaus ^[5] ; Mazza Rodrigues, Jorge L. ^{[6, 7]} ; Tsai, Siu M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Cell & Mol Biol Lab, Piracicaba - Brazil

^[2] Univ Sao Paulo, Oceanog Inst, Dept Biol Oceanog, Sao Paulo - Brazil

^[3] Fed Univ Western Para, Ctr Interdisciplinary Format, Santarem - Brazil

^[4] Univ Oregon, Inst Ecol & Evolut, Dept Biol, Eugene, OR 97403 - USA

^[5] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 - USA

^[6] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 - USA

^[7] Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA - USA

• Tipo de documento: Artigo Científico
• Fonte: Molecular Ecology; v. 30, n. 11, p. 2560-2572, JUN 2021.
• Citações Web of Science: 1

Resumo

The Amazonian floodplain forests are dynamic ecosystems of great importance for the regional hydrological and biogeochemical cycles and function as a significant CH4 source contributing to the global carbon balance. Unique geochemical factors may drive the microbial community composition and, consequently, affect CH4 emissions across floodplain areas. Here, we report the in situ composition of CH4 cycling microbial communities in Amazonian floodplain sediments. We considered how abiotic factors may affect the microbial community composition and, more specifically, CH4 cycling groups. We collected sediment samples during wet and dry seasons from three different types of floodplain forests, along with upland forest soil samples, from the Eastern Amazon, Brazil. We used high-resolution sequencing of archaeal and bacterial 16S rRNA genes combined with real-time PCR to quantify Archaea and Bacteria, as well as key functional genes indicative of the presence of methanogenic (mcrA) and methanotrophic (pmoA) microorganisms. Methanogens were found to be present in high abundance in floodplain sediments, and they seem to resist the dramatic environmental changes between flooded and nonflooded conditions. Methanotrophs known to use different pathways to oxidise CH4 were detected, including anaerobic archaeal and bacterial taxa, indicating that a wide metabolic diversity may be harboured in this highly variable environment. The floodplain environmental variability, which is affected by the river origin, drives not only the sediment chemistry but also the composition of the microbial communities. These environmental changes seem also to affect the pools of methanotrophs occupying distinct niches. Understanding these shifts in the methanotrophic communities could improve our comprehension of the CH4 emissions in the region. (AU)

• Processo FAPESP: 14/50320-4 - Dimensões US-BIOTA - São Paulo: pesquisa colaborativa: integrando as dimensões da biodiversidade microbiana ao longo de áreas de alteração do uso da terra em florestas tropicais
• Beneficiário: Tsai Siu Mui
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 17/26138-0 - Avaliações taxonômica e funcional da comunidade microbiana envolvidas na ciclagem do metano nos solos da Amazônia Brasileira
• Beneficiário: Fabiana da Silva Paula
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/14974-0 - Dinâmica das comunidades microbianas associadas aos ciclos do metano e enxofre em solos amazônicos
• Beneficiário: Júlia Brandão Gontijo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/09643-2 - Comunidades microbianas do solo na conversão floresta-pastagem: uma abordagem multi-ômica
• Beneficiário: Andressa Monteiro Venturini
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/13546-7 - Capacidade de resiliência de comunidades microbianas avaliada por metagenômica e metatranscriptômica em solos de floresta e pastagem da Amazônia
• Beneficiário: Andressa Monteiro Venturini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/25931-3 - Avaliação taxonômica e funcional da comunidade microbiana envolvida na ciclagem do metano em solos da Amazônia Brasileira
• Beneficiário: Andressa Monteiro Venturini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado