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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Prevention of Prostate Cancer in Transgenic Adenocarcinoma of the Mouse Prostate Mice by Yellow Passion Fruit Extract and Antiproliferative Effects of Its Bioactive Compound Piceatannol

Texto completo
Autor(es):
Kido, Larissa Akemi [1, 2] ; Hahm, Eun-Ryeong [3] ; Kim, Su-Hyeong [3] ; Baseggio, Andressa Mara [2] ; Cagnon, Valeria Helena Alves [1] ; Singh, Shivendra V. [3, 4] ; Marostica, Jr., Mario Roberto [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas - Brazil
[2] Univ Estadual Campinas, Fac Food Engn, Dept Food & Nutr, Campinas - Brazil
[3] Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15260 - USA
[4] Univ Pittsburgh, Sch Med, UPMC Hillman Canc Ctr, Pittsburgh, PA 15260 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF CANCER PREVENTION; v. 25, n. 2, p. 87-99, JUN 2020.
Citações Web of Science: 3
Resumo

Piceatannol (PIC), a polyphenol presents in many vegetables and fruits including yellow passion fruit extract (PFE; Passiflora edulis), has anti-cancer activity, but its molecular targets are still poorly understood. The aims of this study were to investigate the molecular mechanistic actions of PIC in prostate cancer cell lines and to test if the extract from PFE rich in PIC can affect the growth of prostate cancer cells in the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model. The PC-3, 22Rv1, LNCaP, and VCaP prostate cancer cells were exposed to PIC (10-40 mu M) and cell viability, lactate measurement, Western blot, and flow cytometric analyses were performed. For an in vivo experiments, eight-week-old TRAMP mice (n = 10 per group each) received an aqueous extract of PFE containing 20 mg of PIC/kg or water (control group) by gavage for 4 or 10 weeks for further analyses. PIC treatment concentration- and time-dependently reduced viability of all cell lines tested. 22Rv1 and LNCaP cells treated with PIC did not exhibit any significant alteration in the intracellular accumulation of lactate. PIC treatment caused G(0)/G(1) phase cell cycle arrest and induction of apoptosis in both LNCaP and 22Rv1 cells. PIC-treated cells exhibited altered protein levels of p53, p21, cyclin D1, and cyclin-dependent kinase 4 (cdk4). The short and long-term PFE treatments also affected p21, cyclin D1 and cdk4 and delayed disease progression in TRAMP, with a decreased incidence of preneoplastic lesions. In conclusion, PIC apparently does not alter glucose metabolism in prostate cancer cells, while cell cycle arrest and p53 modulation are likely important in anti-cancer effects of PIC alone or as a food matrix byproduct in prostate cancer cells, especially those with an androgen-dependent phenotype. (AU)

Processo FAPESP: 18/11069-5 - Ação de berries brasileiras sobre parâmetros metabólicos e desempenho cognitivo de animais obesos
Beneficiário:Mário Roberto Maróstica Junior
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/13320-9 - Efeito do azeite da polpa e do chá das folhas do abacate da variedade Hass associado à atividade física no tratamento da obesidade induzida por dieta hiperlipídica
Beneficiário:Mário Roberto Maróstica Junior
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/50333-1 - Cobra: nova plataforma de descoberta de compostos para identificação rápida e de baixo custo de bioativo com efeitos benéficos originários de plantas brasileiras
Beneficiário:Glaucia Maria Pastore
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 18/09793-7 - Estudo comparativo baseado no ação isolada do piceatannol e como parte do conteúdo bioativo da extrato do bagaço do maracujá na terapia do câncer de próstata
Beneficiário:Larissa Akemi Kido de Barros
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado