Targeted analysis of eicosanoids derived from cytochrome P450 pathway by high-resolution multiple-reaction monitoring mass spectrometry

Autor(es)

Acunha, Tanize ^[1] ; Nardini, Viviani ^[1] ; Ferranti Peti, Ana Paula ^[1] ; Borges Prado, Morgana Kelly ^[1] ; Beraldo Moraes, Luiz Alberto ^[2] ; Faccioli, Lucia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Av Cafe S-N, BR-14040903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Philosophy Sci & Letters Ribeirao Preto, Dept Chem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Mass Spectrometry; v. 56, n. 7 JUL 2021.
• Citações Web of Science: 0

Resumo

Cytochrome P450 (CYP450) pathway is one of the critical enzymatic via eicosanoid biosynthesis. Nevertheless, their metabolites are far less explored. This pathway plays a crucial role in converting arachidonic acid to hydroxyeicosatetraenoic (HETEs), epoxyeicosatrienoic (EETs), dihydroxyeicosatetraenoic acids (DiHETEs), and dihydroxyeicosatrienoic acids (DiHETrEs), which mediate several physiological and pathological functions. However, CYP450-derived eicosanoids are structurally complex, making those analyses a challenge in lipidomics studies. Herein, a high-resolution multiple-reaction monitoring (MRMHR) method has been proposed as a powerful tool for the simultaneous analysis of CYP450-eicosanoids on different biological samples. The developed liquid chromatography (LC)-MRMHR method was partially validated according to the Food and Drug Administration (FDA) criteria, demonstrating adequate specificity, linearity, precision, and accuracy. Besides, several biological samples were analyzed to guarantee the feasibility of the method. The proposed strategy may improve the understanding of CYP450-derived eicosanoids in biological systems, which could be fundamental to reveal new aspects of those in physiologic and pathologic conditions. (AU)

• Processo FAPESP: 16/16788-4 - Identificação e quantificação simultânea de eicosanóides derivados da via do citocromo-P450 (CYP450) utilizando a cromatografia líquida de alta eficiência acoplada à espectrometria de massas sequencial (HPLC-MS/MS)
• Beneficiário: Ana Paula Ferranti Peti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/00658-1 - Novos aspectos funcionais dos eicosanóides
• Beneficiário: Lúcia Helena Faccioli
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 14/07125-6 - Novos aspectos funcionais dos eicosanóides
• Beneficiário: Lúcia Helena Faccioli
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/25704-4 - Análise lipidômica empregando espectrometria de mobilidade iônica diferencial (DMS) para determinação de perfil de lipídeos em peçonhas de escorpiões e serpentes
• Beneficiário: Tanize dos Santos Acunha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado