Targeted analysis of eicosanoids derived from cytochrome P450 pathway by high-resolution multiple-reaction monitoring mass spectrometry

Author(s)

Acunha, Tanize ^[1] ; Nardini, Viviani ^[1] ; Ferranti Peti, Ana Paula ^[1] ; Borges Prado, Morgana Kelly ^[1] ; Beraldo Moraes, Luiz Alberto ^[2] ; Faccioli, Lucia Helena ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Av Cafe S-N, BR-14040903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Philosophy Sci & Letters Ribeirao Preto, Dept Chem, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Mass Spectrometry; v. 56, n. 7 JUL 2021.
• Web of Science Citations: 0

Abstract

Cytochrome P450 (CYP450) pathway is one of the critical enzymatic via eicosanoid biosynthesis. Nevertheless, their metabolites are far less explored. This pathway plays a crucial role in converting arachidonic acid to hydroxyeicosatetraenoic (HETEs), epoxyeicosatrienoic (EETs), dihydroxyeicosatetraenoic acids (DiHETEs), and dihydroxyeicosatrienoic acids (DiHETrEs), which mediate several physiological and pathological functions. However, CYP450-derived eicosanoids are structurally complex, making those analyses a challenge in lipidomics studies. Herein, a high-resolution multiple-reaction monitoring (MRMHR) method has been proposed as a powerful tool for the simultaneous analysis of CYP450-eicosanoids on different biological samples. The developed liquid chromatography (LC)-MRMHR method was partially validated according to the Food and Drug Administration (FDA) criteria, demonstrating adequate specificity, linearity, precision, and accuracy. Besides, several biological samples were analyzed to guarantee the feasibility of the method. The proposed strategy may improve the understanding of CYP450-derived eicosanoids in biological systems, which could be fundamental to reveal new aspects of those in physiologic and pathologic conditions. (AU)

• FAPESP's process: 16/16788-4 - Simultaneous identification and quantification of cytochrome-P450 pathway (CYP450)-derived eicosanoids using high performance liquid chromatography coupled to sequential mass spectrometry (HPLC-MS/MS)
• Grantee: Ana Paula Ferranti Peti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/00658-1 - New functional aspects of eicosanoids
• Grantee: Lúcia Helena Faccioli
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 14/07125-6 - New functional aspects of eicosanoids
• Grantee: Lúcia Helena Faccioli
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/25704-4 - Lipid analysis using ion mobility spectrometry (DMS) for determination of lipid profile in scorpions and snakes venom
• Grantee: Tanize dos Santos Acunha
• Support Opportunities: Scholarships in Brazil - Post-Doctoral