Temporomandibular inflammation regulates the matrix metalloproteinases MMP-2 and MMP-9 in limbic structures

Autor(es)

Nascimento, Glauce C. ^{[1, 2]} ; De Paula, Bruna B. ^[1] ; Gerlach, Raquel F. ^[2] ; Leite-Panissi, Christie R. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Psychol, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Dent Fac, Dept Basic & Oral Biol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. 236, n. 9, p. 6571-6580, SEP 2021.
• Citações Web of Science: 1

Resumo

Temporomandibular disorder (TMD) is characterized by acute or chronic orofacial pain, which can be associated with inflammatory processes in the temporomandibular joint (TMJ) and emotional disorders. Peripheral and central sensitization in painful orofacial processes is common, and it can be triggered by peripheral inflammatory challenge with consequent neuroinflammation phenomena. Such neuroinflammation comes from inflammatory products from supportive cells, blood-brain barrier, and extracellular matrix. Here, we evaluated the possible recruitment of limbic structures for modified matrix metalloproteinases (MMPs) expression and activity during temporomandibular inflammation-induced orofacial persistent pain. The inflammatory process in TMJs of rats was induced by Freund's Complete Adjuvant (CFA) administration. The activity and expression of MMPs-2 and 9 were assessed by in situ zymography and conventional zymography, respectively. A glial colocalization with the MMPs was performed using immunofluorescence. The results evidenced both short- and long-term alterations on MMP-2 and -9 expression in the limbic structures following CFA-induced temporomandibular inflammation. The gelatinolytic activity was increased in the central amygdala, hippocampus, hypothalamus, ventrolateral periaqueductal gray (vlPAG), superior colliculus, and inferior colliculus. Finally, an increase of colocalization of MMP-2/GFAP and MMP-9/GFAP in CFA-induced inflammation groups was observed when compared with saline groups in the central amygdala and vlPAG. It is possible to suggest that glial activation is partly responsible for the production of gelatinases in the persistent orofacial pain, and it is involved in the initiation and maintenance of this process, indicating that inhibition of MMPs might be pursued as a potential new therapeutic target for TMD. (AU)

• Processo FAPESP: 11/07718-9 - Análise comportamental de ratos portadores de inflamação persistente da articulação temporomandibular: correlação com a quantificação de metaloproteinases em estruturas do sistema cerebral aversivo
• Beneficiário: Glauce Crivelaro Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/23888-0 - Estudos de mecanismos de ativação e ação das MMP2 e ADAM17: identificação de proteínas reguladoras, vias de produção de oxidantes, epigenética e alvos proteolíticos
• Beneficiário: Raquel Fernanda Gerlach
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/11213-6 - Envolvimento do monóxido de carbono na modulação do comportamento emocional de ratos com endotoxemia.
• Beneficiário: Christie Ramos Andrade Leite Panissi
• Modalidade de apoio: Auxílio à Pesquisa - Regular