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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Temporomandibular inflammation regulates the matrix metalloproteinases MMP-2 and MMP-9 in limbic structures

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Author(s):
Nascimento, Glauce C. [1, 2] ; De Paula, Bruna B. [1] ; Gerlach, Raquel F. [2] ; Leite-Panissi, Christie R. A. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Psychol, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Dent Fac, Dept Basic & Oral Biol, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Cellular Physiology; v. 236, n. 9, p. 6571-6580, SEP 2021.
Web of Science Citations: 1
Abstract

Temporomandibular disorder (TMD) is characterized by acute or chronic orofacial pain, which can be associated with inflammatory processes in the temporomandibular joint (TMJ) and emotional disorders. Peripheral and central sensitization in painful orofacial processes is common, and it can be triggered by peripheral inflammatory challenge with consequent neuroinflammation phenomena. Such neuroinflammation comes from inflammatory products from supportive cells, blood-brain barrier, and extracellular matrix. Here, we evaluated the possible recruitment of limbic structures for modified matrix metalloproteinases (MMPs) expression and activity during temporomandibular inflammation-induced orofacial persistent pain. The inflammatory process in TMJs of rats was induced by Freund's Complete Adjuvant (CFA) administration. The activity and expression of MMPs-2 and 9 were assessed by in situ zymography and conventional zymography, respectively. A glial colocalization with the MMPs was performed using immunofluorescence. The results evidenced both short- and long-term alterations on MMP-2 and -9 expression in the limbic structures following CFA-induced temporomandibular inflammation. The gelatinolytic activity was increased in the central amygdala, hippocampus, hypothalamus, ventrolateral periaqueductal gray (vlPAG), superior colliculus, and inferior colliculus. Finally, an increase of colocalization of MMP-2/GFAP and MMP-9/GFAP in CFA-induced inflammation groups was observed when compared with saline groups in the central amygdala and vlPAG. It is possible to suggest that glial activation is partly responsible for the production of gelatinases in the persistent orofacial pain, and it is involved in the initiation and maintenance of this process, indicating that inhibition of MMPs might be pursued as a potential new therapeutic target for TMD. (AU)

FAPESP's process: 11/07718-9 - Behavioral analysis of rats with persistent inflammation of the temporomandibular joint: correlation with the quantification of metalloproteinases in the structures of the brain aversive system
Grantee:Glauce Crivelaro Do Nascimento
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/23888-0 - Studies on the activation mechanisms of MMP-2 and ADAM17: identification of regulatory proteins, oxidant production pathways, epigenetics and proteolytic targets
Grantee:Raquel Fernanda Gerlach
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/11213-6 - Involvement of carbon monoxide in modulation of emotional behavior in endotoxemic rats.
Grantee:Christie Ramos Andrade Leite Panissi
Support type: Regular Research Grants