xposure of Physalaemus cuvieri (Anura) to benzo[a]pyrene and alpha-naphthoflavone: Morphofunctional effects on hepatic melanomacrophages and erythrocytes abnormalitie

Autor(es)

Fanali, Lara Zacari ^[1] ; Sturve, Joachim ^[2] ; de Oliveira, Classius ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Grad Program Anim Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Gothenburg, Dept Biol & Environm Sci, Box 463, S-40530 Gothenburg - Sweden

^[3] Univ Estadual Paulista Unesp, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Pollution; v. 285, SEP 15 2021.
• Citações Web of Science: 0

Resumo

Benzo{[}a]pyrene (BaP) is a high-risk contaminant of elevated toxicity. Its biotransformation process occurs as the expression of CYP1A1 increases and produces toxic metabolites. In turn, alpha-naphthoflavone (aNF) represents an inhibitor of CYP1A1, preventing BaP metabolism. Toxicological studies in anurans show alterations in the melanomacrophage (MM) detoxification cell after exposure to xenobiotics. In this study, the production of melanin by MMs was evaluated, as were morphological alterations in the cytoskeleton, phagocytosis and the genotoxicity effects after exposure of an anuran species to BaP and aNF. Physalaemus cuvieri received subcutaneous injections of 2 mg/kg and/or 20 mg/kg aNF. For phagocytosis analyses, animals received an intraperitoneal injection with 0.4% trypan blue. The results revealed that melanin synthesis increased by 503.2% in animals exposed to BaP after 48 h, which was related to the antioxidant action of melanin, whereas the decreased in synthesis of 25.6% with the BaP + aNF interaction resulted in high toxicity to MMs and cell degeneration. The phagocytic activity reduced to 37.6% in animals exposed to BaP, characterizing a functional impairment; however, the BaP + aNF interaction led to the restoration of phagocytosis, reaching 419.23%. The decreased rate or absence of abnormalities may be explained by the fact that only the less damaged erythrocytes remained in the bloodstream, whereas the most damaged cells died. In conclusion, BaP and aNF are toxic to P. cuvieri, bringing risks to herpetofauna. (AU)

• Processo FAPESP: 18/01078-7 - O fígado de anfíbios anuros: morfologia comparada entre 10 espécies e ação do benzopireno nos melanomacrófagos de Physalaemus cuvieri
• Beneficiário: Classius de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/07971-2 - Alterações morfofuncionais nos melanomacrófagos hepáticos de Physalaemus cuvieri (Anura: Leptodactylidae) induzidas pelo contaminante benzo[alfa]pireno.
• Beneficiário: Lara Zácari Fanali
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/02067-5 - Efeitos tóxicos dos herbicidas Atrazina e Roundup em Eupemphix nattereri (Leiuperidae) e Rhinella schneideri (Bufonidae): alterações morfológicas sob diferentes concentrações e condições abióticas (pH, temperatura e fotoperíodo)
• Beneficiário: Classius de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular