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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

xposure of Physalaemus cuvieri (Anura) to benzo[a]pyrene and alpha-naphthoflavone: Morphofunctional effects on hepatic melanomacrophages and erythrocytes abnormalitie

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Author(s):
Fanali, Lara Zacari [1] ; Sturve, Joachim [2] ; de Oliveira, Classius [3]
Total Authors: 3
Affiliation:
[1] Univ Estadual Paulista UNESP, Grad Program Anim Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Gothenburg, Dept Biol & Environm Sci, Box 463, S-40530 Gothenburg - Sweden
[3] Univ Estadual Paulista Unesp, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Environmental Pollution; v. 285, SEP 15 2021.
Web of Science Citations: 0
Abstract

Benzo{[}a]pyrene (BaP) is a high-risk contaminant of elevated toxicity. Its biotransformation process occurs as the expression of CYP1A1 increases and produces toxic metabolites. In turn, alpha-naphthoflavone (aNF) represents an inhibitor of CYP1A1, preventing BaP metabolism. Toxicological studies in anurans show alterations in the melanomacrophage (MM) detoxification cell after exposure to xenobiotics. In this study, the production of melanin by MMs was evaluated, as were morphological alterations in the cytoskeleton, phagocytosis and the genotoxicity effects after exposure of an anuran species to BaP and aNF. Physalaemus cuvieri received subcutaneous injections of 2 mg/kg and/or 20 mg/kg aNF. For phagocytosis analyses, animals received an intraperitoneal injection with 0.4% trypan blue. The results revealed that melanin synthesis increased by 503.2% in animals exposed to BaP after 48 h, which was related to the antioxidant action of melanin, whereas the decreased in synthesis of 25.6% with the BaP + aNF interaction resulted in high toxicity to MMs and cell degeneration. The phagocytic activity reduced to 37.6% in animals exposed to BaP, characterizing a functional impairment; however, the BaP + aNF interaction led to the restoration of phagocytosis, reaching 419.23%. The decreased rate or absence of abnormalities may be explained by the fact that only the less damaged erythrocytes remained in the bloodstream, whereas the most damaged cells died. In conclusion, BaP and aNF are toxic to P. cuvieri, bringing risks to herpetofauna. (AU)

FAPESP's process: 18/01078-7 - The liver of anuran amphibians: morphology compared between 10 species and action of benzopyrene in the melanomacrophages of Physalaemus cuvieri
Grantee:Classius de Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 17/07971-2 - Morphofunctional changes in hepatic melanomacrophages of Physalaemus cuvieri (Anura: Leptodactylidae) induced by the contaminant benzo[alpha]pyrene.
Grantee:Lara Zácari Fanali
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/02067-5 - Toxic effects of the herbicides Atrazine and Roundup in Eupemphix nattereri (Leiuperidae) and Rhinella schneideri (Bufonidae): morphological changes under different concentrations and abiotic conditions (pH, temperature and photoperiod)
Grantee:Classius de Oliveira
Support Opportunities: Regular Research Grants