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Effects of high temperature on visceral pigmentation and male germinative epithelium in Eupemphix nattereri and Leptodactylus fuscus (Anura)

Grant number: 14/16900-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): November 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Biological Sciences - Zoology - Morphology of Recent Groups
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Classius de Oliveira
Grantee:Gabriela Baroni Leite
Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil


Temperature influences aspects physiological and morphological in animals ectothermic. In addition, anurans have skin permeability and cycle of life dependent of the environmental aquatic and terrestrial, which makes them vulnerable to environmental changes. Anurans have an extracutaneous pigmentary system, comprising melanocytes and melanomacrophages, which responds to temperature changes, suggesting thermal protection role of these cells. Besides, changes in temperature can decrease the reproductive performance these animals, interfering in spermatogenesis and consequently reproductive success of these animals. High temperatures may also have genotoxic effects, generating micronuclei in erythrocytes. This characteristic is used as biomarkers of genetic damage arising from the environmental changes. Therefore, we aim to evaluate the systemic effects of the high temperature in Eupemphix nattereri and Leptodactylus fuscus, seeking evidence about the function of the melanomacrophages, genotoxic effects and effects in germ cells. For that, will be used 56 animals being 28 specimens of each species divided into four experimental groups (n=7) being a control group and the others exposed to a temperature of 35.1°C in B.O.D chamber with photoperiod 12 light/12 dark for 5, 10 and 15 days. Subsequently the animals will be anesthetized and blood samples will be taken for micronucleos analysis. After this procedure the animals will be euthanized and removed fragments of liver and testes for analysis of liver melanomacrophages, testicular melanocytes and germ cells. With these analyzes we seek to evaluate the responses of various tissues due to heat stress and thus elucidate possible effects of high temperature on native species and evaluating genotoxic effects on metabolic and reproductive aspects. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LEITE, GABRIELA BARONI; FRANCO-BELUSSI, LILIAN; PROVETE, DIOGO B.; DE OLIVEIRA, CLASSIUS. Comparative testis morphology of Neotropical anurans. ZOOLOGISCHER ANZEIGER, v. 257, p. 29-38, . (14/16900-3, 14/00946-4, 13/02067-5, 13/07284-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LEITE, Gabriela Baroni. Effects of high temperature on visceral pigmentation and male germinative epithelium in Physalaemus nattereri and Leptodactylus fuscus (Anura). 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.

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