Toxic effects of the herbicides Atrazine and Roundup in Eupemphix nattereri (Leiuperidae) and Rhinella schneideri (Bufonidae): morphological changes under different concentrations and abiotic conditions (pH, temperature and photoperiod)

• Grant number: 13/02067-5
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2013
• End date: May 31, 2015
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Classius de Oliveira
• Grantee: Classius de Oliveira
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

We expect to detect and describe the effects of the herbicide formulated glyphosate and atrazine for larvae Eupemphix nattereri (Leiuperidae) and Rhinella schneideri (Bufonidae). Using appropriate experimental designs we want to have information to evaluate and prove the toxicity of products (environmental contaminants / endocrine disruptors) as used in our region (northwest of São Paulo) and in our current model of agriculture in Brazil. In addition, we also expect to evaluate the susceptibility of these different species to pesticides and demonstrate the interaction of abiotic factors with the use of these compounds, defending the hypothesis that changes in environmental conditions can affect its toxicity. Specifically, we know that the herbicides exhibit toxicity to organisms at different levels. These substances are inducing malformations in larvae and morphophysiological systemic changes. We believe that for the gonads will interfere with normal development of individuals In Brazil there is no data on these contaminants and, as a result of abusive use of pesticides are probably affecting reproduction of the species. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FANALI, LARA ZACARI; DE OLIVEIRA, CLASSIUS; STURVE, JOACHIM. Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss). Environmental Science and Pollution Research, v. 28, n. 38, p. 53926-53935, OCT 2021. (18/07989-1, 13/02067-5, 18/01078-7)

BORGES, RINNEU ELIAS; DE SOUZA SANTOS, LIA RAQUEL; BENVINDO-SOUZA, MARCELINO; MODESTO, RICHELLE SOUSA; ASSIS, RHAYANE ALVES; DE OLIVEIRA, CLASSIUS. Genotoxic Evaluation in Tadpoles Associated with Agriculture in the Central Cerrado, Brazil. Archives of Environmental Contamination and Toxicology, v. 77, n. 1, p. 22-28, JUL 2019. (13/02067-5)

GREGORIO, L. S.; FREITAS, J. S.; FRANCO-BELUSSI, L.; DE OLIVEIRA, C.. LPS-induced alterations in reproductive organs and liver pigmentation in the toad Rhinella diptycha (Bufonidae). CANADIAN JOURNAL OF ZOOLOGY, v. 96, n. 12, p. 1336-1345, DEC 2018. (14/00946-4, 11/21519-9, 13/02067-5, 13/16948-3)

MANUEL PEREZ-IGLESIAS, JUAN; FRANCO-BELUSSI, LILIAN; SEBASTIAN NATALE, GUILLERMO; DE OLIVEIRA, CLASSIUS. Biomarkers at different levels of organisation after atrazine formulation (SIPTRAN 500SC (R)) exposure in Rhinella schineideri (Anura: Bufonidae) Neotropical tadpoles. Environmental Pollution, v. 244, p. 733-746, JAN 2019. (13/02067-5, 14/00946-4)

BORGES, RINNEU ELIAS; DE SOUZA SANTOS, LIA RAQUEL; ASSIS, RHAYANE ALVES; BENVINDO-SOUZA, MARCELINO; FRANCO-BELUSSI, LILIAN; DE OLIVEIRA, CLASSIUS. Monitoring the morphological integrity of neotropical anurans. Environmental Science and Pollution Research, v. 26, n. 3, p. 2623-2634, JAN 2019. (13/02067-5)

LEITE, GABRIELA BARONI; FRANCO-BELUSSI, LILIAN; PROVETE, DIOGO B.; DE OLIVEIRA, CLASSIUS. Comparative testis morphology of Neotropical anurans. ZOOLOGISCHER ANZEIGER, v. 257, p. 29-38, 2015. (14/16900-3, 14/00946-4, 13/02067-5, 13/07284-4)

DE SOUZA SANTOS, LIA RAQUEL; FRANCO-BELUSSI, LILIAN; ZIERI, RODRIGO; BORGES, RINNEU ELIAS; DE OLIVEIRA, CLASSIUS. Effects of Thermal Stress on Hepatic Melanomacrophages of Eupemphix nattereri (Anura). Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. 297, n. 5, p. 864-875, MAY 2014. (05/02919-5, 11/01840-7, 13/02067-5)

FANALI, LARA ZACARI; STURVE, JOACHIM; DE OLIVEIRA, CLASSIUS. xposure of Physalaemus cuvieri (Anura) to benzo[a]pyrene and alpha-naphthoflavone: Morphofunctional effects on hepatic melanomacrophages and erythrocytes abnormalitie. Environmental Pollution, v. 285, SEP 15 2021. (18/01078-7, 17/07971-2, 13/02067-5)

FANALI, LARA ZACARI; DE OLIVEIRA, CLASSIUS; STURVE, JOACHIM. Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss). Environmental Science and Pollution Research, MAY 2021. (18/01078-7, 13/02067-5, 18/07989-1)

GREGORIO, L. S.; FRANCO-BELUSSI, L.; DE OLIVEIRA, C.. Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura) tadpoles and juveniles. Environmental Pollution, v. 251, p. 879-884, AUG 2019. (14/00946-4, 13/02067-5, 18/01078-7)

FRANCO-BELUSSI, L.; DE OLIVEIRA, C.. The spleen of Physalaemus nattereri (Amphibia: Anura): morphology, melanomacrophage pigment compounds and responses to -melanocyte stimulating hormone. ITALIAN JOURNAL OF ZOOLOGY, v. 83, n. 3, p. 298-305, SEP 2016. (14/00946-4, 13/02067-5, 11/01840-7, 15/12006-9)

FANALI, LARA ZACARI; DE LACERDA VALVERDE, BRUNO SERRA; FRANCO-BELUSSI, LILIAN; PROVETE, DIOGO B.; DE OLIVEIRA, CLASSIUS. Response of digestive organs of Hypsiboas albopunctatus (Anura: Hylidae) to benzo[alpha]pyrene. Amphibia-Reptilia, v. 38, n. 2, p. 175-185, 2017. (13/18469-5, 13/02067-5, 13/05907-4, 14/00946-4)

DE GREGORIO, LARA S.; FRANCO-BELUSSI, LILIAN; GOMES, FERNANDO R.; DE OLIVEIRA, CLASSIUS. Flutamide effects on morphology of reproductive organs and liver of Neotropical Anura, Rhinella schneideri. AQUATIC TOXICOLOGY, v. 176, p. 181-189, JUL 2016. (14/00946-4, 13/02067-5, 11/01840-7)

MANUEL PEREZ-IGLESIAS, JUAN; FRANCO-BELUSSI, LILIAN; MORENO, LILIANA; TRIPOLE, SUSANA; DE OLIVEIRA, CLASSIUS; SEBASTIAN NATALE, GUILLERMO. Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus. Environmental Science and Pollution Research, v. 23, n. 10, p. 9852-9861, MAY 2016. (13/02067-5, 14/00946-4)

FRANCO-BELUSSI, LILIAN; LEITE, GABRIELA BARONI; FREITAS, JULIANE SILBERSCHMIDT; DE OLIVEIRA, CLASSIUS. Morphological effects of bacterial compounds on the testes of Eupemphix nattereri (Anura). ANIMAL BIOLOGY, v. 64, n. 3, p. 261-275, 2014. (09/13925-7, 13/02067-5, 11/01840-7)

FRANCO-BELUSSI, LILIAN; PROVETE, DIOGO B.; BORGES, RINNEU E.; DE OLIVEIRA, CLASSIUS; SANTOS, LIA RAQUEL S.. Idiosyncratic liver pigment alterations of five frog species in response to contrasting land use patterns in the Brazilian Cerrado. PeerJ, v. 8, AUG 26 2020. (14/00946-4, 13/02067-5, 18/01078-7)