Effects of benzo[a]pyrene on the cytoskeleton and melanin dispersion of hepatic melanomacrophages of fish and amphibians

• Grant number: 18/07989-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 10, 2018
• End date: March 10, 2019
• Field of knowledge: Biological Sciences - Zoology - Morphology of Recent Groups
• Principal Investigator: Classius de Oliveira
• Grantee: Lara Zácari Fanali
• Supervisor: Erik Lennart Joachim Sturve
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Institution abroad: University of Gothenburg, Sweden
• Associated to the scholarship: 17/07971-2 - Morphofunctional changes in hepatic melanomacrophages of Physalaemus cuvieri (Anura: Leptodactylidae) induced by the contaminant benzo[alpha]pyrene., BP.DR

Abstract

Benzo[a]pyrene (BaP) is a toxic compound present in the environment and capable to affect fish and amphibians. The liver of these vertebrates have cells with detoxification function called melanomacrophages (MMs). A characteristic of MMs is the capacity of produce and store melanin, a protection pigment that is able to eliminate free radicals and neutralizing cations, thereby protecting tissues from cytotoxic damage. Melanin granules can aggregate/disperse with the use of cytoskeleton components (microtubules and actin filaments), but contaminants are able to influence the cytoskeleton and compromise this motility of melanin granules, which might hinder the detoxification function of the MMs. According to the hypothesis that BaP is genotoxic, affects the melanin dispersion and the microtubules, our goals will be: to evaluate BaP changes in the cytoskeleton; evaluate dispersion of the melanin area of the hepatic MMs and evaluate genotoxic effects on the erythrocytes. The experimental times will be 48 hours and 7 days, where the animals will receive doses 2mg/kg of BaP diluted in mineral oil, while the control groups will receive only mineral oil. For microtubules, pellets with MMs will be analyzed under a fluorescence microscope. For the histological analysis, the procedures for inclusion in historesin and light microscopy analysis of the melanin area will be followed. For nuclear abnormalities the blood will be extracted and the procedures for analysis will be followed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FANALI, LARA ZACARI; DE OLIVEIRA, CLASSIUS; STURVE, JOACHIM. Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss). Environmental Science and Pollution Research, v. 28, n. 38, p. 53926-53935, OCT 2021. (18/07989-1, 13/02067-5, 18/01078-7)

FANALI, LARA ZACARI; DE OLIVEIRA, CLASSIUS; STURVE, JOACHIM. Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss). Environmental Science and Pollution Research, MAY 2021. (18/01078-7, 13/02067-5, 18/07989-1)