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Effects of benzo[a]pyrene on the cytoskeleton and melanin dispersion of hepatic melanomacrophages of fish and amphibians

Grant number: 18/07989-1
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 10, 2018
Effective date (End): March 10, 2019
Field of knowledge:Biological Sciences - Zoology
Principal Investigator:Classius de Oliveira
Grantee:Lara Zácari Fanali
Supervisor abroad: Erik Lennart Joachim Sturve
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
Local de pesquisa : University of Gothenburg, Sweden  
Associated to the scholarship:17/07971-2 - Morphofunctional changes in hepatic melanomacrophages of Physalaemus cuvieri (Anura: Leptodactylidae) induced by the contaminant benzo[alpha]pyrene., BP.DR

Abstract

Benzo[a]pyrene (BaP) is a toxic compound present in the environment and capable to affect fish and amphibians. The liver of these vertebrates have cells with detoxification function called melanomacrophages (MMs). A characteristic of MMs is the capacity of produce and store melanin, a protection pigment that is able to eliminate free radicals and neutralizing cations, thereby protecting tissues from cytotoxic damage. Melanin granules can aggregate/disperse with the use of cytoskeleton components (microtubules and actin filaments), but contaminants are able to influence the cytoskeleton and compromise this motility of melanin granules, which might hinder the detoxification function of the MMs. According to the hypothesis that BaP is genotoxic, affects the melanin dispersion and the microtubules, our goals will be: to evaluate BaP changes in the cytoskeleton; evaluate dispersion of the melanin area of the hepatic MMs and evaluate genotoxic effects on the erythrocytes. The experimental times will be 48 hours and 7 days, where the animals will receive doses 2mg/kg of BaP diluted in mineral oil, while the control groups will receive only mineral oil. For microtubules, pellets with MMs will be analyzed under a fluorescence microscope. For the histological analysis, the procedures for inclusion in historesin and light microscopy analysis of the melanin area will be followed. For nuclear abnormalities the blood will be extracted and the procedures for analysis will be followed. (AU)