Photobiomodulation inhibits inflammation in the temporomandibular joint of rats

Autor(es)

Pereira, Eliana de Souza Bastos Mazuqueli ^[1] ; Basting, Rosanna Tarkany ^[1] ; Abdalla, Henrique Ballassini ^[1] ; Garcez, Aguinaldo Silva ^[1] ; Napimoga, Marcelo Henrique ^[1] ; Clemente-Napimoga, Juliana Trindade ^[1]

Afiliação do(s) autor(es)

^[1] Sao Leopoldo Mandic Inst & Res Ctr, Lab Neuroimmune Interface Pain Res, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 222, SEP 2021.
• Citações Web of Science: 0

Resumo

Photobiomodulation (PBM) has been applied as a non-invasive technique for treating temporomandibular joint symptoms, especially on painful condition's relief, however the anti-inflammatory mechanism underlying the effect of PBM remains uncertain. This study aims to evaluate the mechanisms of action of PBM (808 nm) in a carrageenan-induced inflammation on temporomandibular joint (TMJ) of rats. In this study male Wistar rats were pre-treated with irradiation of a low-power diode laser for 15 s on TMJ (infra-red 808 nm, 100 mW, 50 J/ cm2 and 1.5 J) 15 min prior an injection in the temporomandibular joint of carrageenan (100 mu g/TMJ). 1 h after the TMJ treatments, the rats were terminally anesthetized for joint cavity wash and periarticular tissues collect. Samples analysis demonstrated that PBM inhibit leukocytes chemotaxis in the TMJ and significantly reduces amounts of TNF-alpha, IL-1I3 and CINC-1. In addition, Western blotting analysis demonstrated that PBM significantly decreased the protein levels of P2X3 and P2X7 receptors in the periarticular tissues. On the other hand, PBM was able to increase protein level of IL-10 (anti-inflammatory cytokine). In summary, it is possible to suggest that PBM inhibit inflammatory chemotaxis, modulation the balance of the pro- and anti-inflammatory characteristics of inflammatory cells. (AU)

• Processo FAPESP: 17/22334-9 - Uso de sistemas de liberação de fármacos para o desenvolvimento e aplicabilidade de agentes anti-inflamatórios com potencial efeito imunomodulador e neuroprotetor
• Beneficiário: Marcelo Henrique Napimoga
• Modalidade de apoio: Auxílio à Pesquisa - Temático