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de Freitas, Carla Longo ^{[1, 2]} ; Polonio, Carolina Manganeli ^{[1, 2]} ; Brandao, Wesley Nogueira ^{[1, 2]} ; Rossato, Cristiano ^{[1, 2]} ; Zanluqui, Nagela Ghabdan ^{[3, 1, 2]} ; de Oliveira, Lilian Gomes ^{[1, 2]} ; de Oliveira, Marilia Garcia ^{[1, 2]} ; Evangelista, Lucila Pires ^{[2, 4]} ; Halpern, Silvio ^{[2, 5]} ; Maluf, Mariangela ^{[2, 6]} ; Czresnia, Carlos Eduardo ^{[2, 4]} ; Perin, Paulo ^{[2, 6]} ; de Almeida, Danilo Candido ^{[2, 7]} ; Schatzmman Peron, Jean Pierre ^{[3, 1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Neuroimmune Interact Lab, Dept Immunol, Av Prof Lineu Prestes, 1730 Lab 232, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sci Platform Pasteur USP, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Immunopathol & Allergy Post Grad Program, Sao Paulo - Brazil

^[4] Celula Mater Clin, Reprod Med Div, Sao Paulo - Brazil

^[5] Halpern Clin, Reprod Med Div, Sao Paulo - Brazil

^[6] CEERH Clin, Reprod Med Div, Sao Paulo - Brazil

^[7] Fed Univ Sao Paulo UNIFESP, Nephrol Div, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: STEM CELL REVIEWS AND REPORTS; AUG 2021.
• Citações Web of Science: 0

Resumo

Mesenchymal stem cells comprise a natural reservoir of undifferentiated cells within adult tissues. Given their self-renewal, multipotency, regenerative potential and immunomodulatory properties, MSCs have been reported as a promising cell therapy for the treatment of different diseases, including neurodegenerative and autoimmune diseases. In this study, we investigated the immunomodulatory properties of human tubal mesenchymal stem cells (htMSCs) using the EAE model. htMSCs were able to suppress dendritic cells activation downregulating antigen presentation-related molecules, such as MHCII, CD80 and CD86, while impairing IFN-gamma and IL-17 and increasing IL-10 and IL-4 secretion. It further correlated with milder disease scores when compared to the control group due to fewer leukocytes infiltrating the CNS, specially Th1 and Th17 lymphocytes, associated with increased IL-10 secreting Tr1 cells. Conversely, microglia were less activated and infiltrating mononuclear cells secreted higher levels of IL-4 and IL-10 and expressed reduced chemokine receptors as CCR4, CCR6 and CCR8. qPCR of the spinal cords revealed upregulation of indoleamine-2,3-dioxygenase (IDO) and brain derived neurotrophic factor (BDNF). Taken together, here evidenced the potential of htMSCs as an alternative for the treatment of inflammatory, autoimmune or neurodegenerative diseases. (AU)

• Processo FAPESP: 20/06145-4 - Imunopatogênese da COVID-19 em modelos experimentais e vacina nasal anti-SARS-CoV-2
• Beneficiário: Jean Pierre Schatzmann Peron
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/07371-2 - Caracterização do papel de linfócitos T CD8+ na infecção por Zika vírus
• Beneficiário: Nagela Ghabdan Zanluqui
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/10242-5 - Papel do receptor ionotrópico de glutamato NMDA em células da imunidade inata e adaptativa da mucosa intestinal em modelo experimental murino
• Beneficiário: Marília Garcia de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/11828-0 - Avaliação do papel dos microRNAs na imunopatogênese da microcefalia causada pelo Zika vírus em modelos experimentais
• Beneficiário: Carolina Manganeli Polonio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/22504-1 - O papel dos receptores TAM e seus ligantes, Gas6 e Pros1, na Síndrome Congênita do Zika Vírus Experimental
• Beneficiário: Jean Pierre Schatzmann Peron
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/26170-0 - Neuroimunobiologia em modelo experimental de Encefalomielite Autoimune e Síndrome Congênita do Zika Vírus: fisiopatogenia, susceptibilidade, terapia celular, vacinação
• Beneficiário: Carolina Demarchi Munhoz
• Modalidade de apoio: Auxílio à Pesquisa - Temático