yclodextrins-in-Liposomes: A Promising Delivery System for Lippia sidoides and Syzygium aromaticum Essential Oil

Autor(es)

Baldim, Iara ^{[1, 2]} ; Oliveira, Andressa M. ^[1] ; Souto, Eliana B. ^[2] ; Oliveira, Wanderley P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Minho, CEB Ctr Biol Engn, Campus Gualtar, P-4710057 Braga - Portugal

• Tipo de documento: Artigo Científico
• Fonte: LIFE-BASEL; v. 12, n. 1 JAN 2022.
• Citações Web of Science: 0

Resumo

Biological activity of essential oils (EOs) has been extensively reported; however, their low aqueous solubility, high photosensitivity, and volatility compromise a broad industrial use of these compounds. To overcome these limitations, we proposed a nanoencapsulation approach to protect EOs, that aims to increase their stability and modulate their release profile. In this study, drug-in-cyclodextrin-in-liposomes encapsulating two essential oils (Lippia sidoides and Syzygium aromaticum) and their respective major compounds (thymol and eugenol) were produced by ethanol injection and freeze-dried to form proliposomes and further physicochemically characterized. Liposomes showed high physical stability over one month of storage at 4 degrees C, with slight changes in the mean size, polydispersity index (PDI), and zeta potential. Reconstituted proliposomes showed a mean size between 350 and 3300 nm, PDI from 0.29 to 0.41, and zeta potential between -22 and -26 mV. Differential scanning calorimetry and X-ray diffraction of proliposomes revealed a less-ordered crystalline structure, leading to high retention of the major bioactive compounds (between 73% and 93% for eugenol, and 74% and 84% for thymol). This work highlights the advantages of using drug-in-cyclodextrin-in-liposomes as delivery systems to retain volatile compounds, increasing their physicochemical stability and their promising potential to be utilized as carriers in products in the pharmaceutical, food, and cosmetic industries. (AU)

• Processo FAPESP: 18/26069-0 - Encapsulação de óleos essenciais para uso em formulações farmacêuticas com atividade antimicrobiana
• Beneficiário: Wanderley Pereira Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular