Simultaneous binding of folic acid and lutein to ?-lactoglobulin and ?-lactalbumin: A spectroscopic and molecular docking study

Autor(es)

Vidotto, Danilo C. ; Tavares, Guilherme M.

• Tipo de documento: Artigo Científico
• Fonte: FOOD BIOSCIENCE; v. 50, p. 9-pg., 2022-12-01.

Resumo

Whey proteins are versatile molecules with great nutritional value and multiple functionalities. One of these functionalities is the ability to bind and protect bioactive compounds. Lutein and folic acid (FA) are bioactive molecules with a remarkable relevance for developing functional foods. FA contributes in preventing the occurrence of neural tube defects during the early stages of life, while lutein is a macular pigment that contributes to the development of the retina. In this context, the aim of this work was to evaluate the ability of the main whey proteins, beta-lactoglobulin and alpha-lactalbumin, to simultaneously bind FA and lutein. The binding was evaluated through intrinsic fluorescence quenching and molecular docking, and the impact of the binding on the secondary structure and denaturation temperature of the proteins were also evaluated through circular dichroism and differential scanning calorimetry, respectively. Both proteins had similar behavior on the performed analyses. The multi-binding ability of beta-lactoglobulin and alpha-lactalbumin to FA and lutein was observed. The presence of the first ligand slightly reduced the association constant between the second one and the proteins; in particular for the binding of lutein in the presence of FA (reduction of around 36%-38% on the KSV values). Molecular docking indicated that both ligands interact, preferentially, on the same region of the studied protein structures. The secondary structure of the proteins, as well as their denaturation temperatures were minimally impacted by the presence of these ligands, themselves. Our results may contribute to the development of multi-functional protein-rich food products. (AU)

• Processo FAPESP: 18/03822-5 - EMU concedido no processo 2017/09214-4: cromatógrafo preparativo - Akta pure (GE healthcare)
• Beneficiário: Guilherme Miranda Tavares
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
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• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
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• Beneficiário: Danilo Corsi Vidotto
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/23752-1 - EMU concedido no processo 2013/07914-8 sistema de cromatografia líquida de alta velocidade acoplado a detectores de arranjo de diodos, fluorescência e massas
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• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários