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Brilliant Blue G, But Not Fenofibrate, Treatment Reverts Hemiparkinsonian Behavior and Restores Dopamine Levels in an Animal Model of Parkinson's Disease

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Autor(es):
Ferrazoli, Eneas G. ; de Souza, Hellio D. N. ; Nascimento, Isis C. ; Oliveira-Giacomelli, Agatha ; Schwindt, Telma T. ; Britto, Luiz R. ; Ulricht, Henning
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: CELL TRANSPLANTATION; v. 26, n. 4, p. 9-pg., 2017-01-01.
Resumo

Parkinson's disease (PD) is a neurodegenerative disorder, characterized by the loss of dopaminergic neurons in the substantia nigra and their projections to the striatum. Several processes have been described as potential inducers of the dopaminergic neuron death, such as inflammation, oxidative stress, and mitochondrial dysfunction. However, the death of dopaminergic neurons seems to be multifactorial, and its cause remains unclear. ATP-activating purinergic receptors influence various physiological functions in the CNS, including neurotransmission. Purinergic signaling is also involved in pathological scenarios, where ATP is extensively released and promotes sustained purinergic P2X7 receptor (P2X7R) activation and consequent induction of cell death. This effect occurs, among other factors, by oxidative stress and during the inflammatory response. On the other hand, peroxisome proliferator-activated receptor-gamma coactivator la (PGC-1 alpha) is involved in energy metabolism and mitochondrial biogenesis. Expression and activity upregulation of this protein has been related with reduction of oxidative stress and neuroprotection. Therefore, P2X7R and PGC-1 alpha are potential targets in the treatment of PD. Here hemiparkinsonism was induced by unilateral stereotactic injection of 6-OHDA in a rat model. After 7 days, the establishment of PD was confirmed and followed by treatment with the P2X7R antagonist Brilliant Blue G (BBG) or PGC-1 alpha agonist fenofibrate. BBG, but not fenofibrate, reverted hemiparkinsonian behavior accompanied by an increase in tyrosine hydroxylase immunoreactivity in the substantia nigra. Our results suggest that the P2X7R may be a therapeutic target in Parkinson's disease. (AU)

Processo FAPESP: 12/20685-5 - Papel do receptor B2 de cininas na terapia da Doença de Parkinson em modelo animal.
Beneficiário:Héllio Danny Nóbrega de Souza
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/18730-0 - Identificação e purificação de células-tronco cancerosas de pulmão por aptâmeros de DNA seguida do estudo do sistema calicreína-cininas na progressão tumoral
Beneficiário:Isis Cristina Corrêa Do Nascimento
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/09852-4 - Utilização de bloqueadores de p2x7r e ativadores de pgc-1± combinados à terapia celular em modelo animal de doença de parkinson.
Beneficiário:Enéas Galdini Ferrazoli
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/50880-4 - Células-tronco: dos papéis de receptores de cininas e purinas às aplicações terapêuticas
Beneficiário:Alexander Henning Ulrich
Modalidade de apoio: Auxílio à Pesquisa - Temático