Effect of exogenous galectin-1 on leukocyte migration: modulation of cytokine levels and adhesion molecules

Autor(es)

Gil, Cristiane D. ^{[1, 2]} ; Gullo, Caio E. ^[2] ; Oliani, Sonia M. ^[3]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, BR-04023900 Sao Paulo - Brazil

^[2] Sao Jose do Rio Preto Sch Med FAMERP, Dept Anat, Sao Jose Do Rio Preto, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY; v. 4, n. 1, p. 74-84, 2011.
• Citações Web of Science: 22

Resumo

The effect of exogenous Gal-1 on cellular response and adhesion molecule expression was investigated in a classical model of acute inflammation induced by zymosan. C57BL6 mice, treated or not with human recombinant (hr) Gal-1, received i.p. injection of zymosan and peritoneal exudate, blood and mesentery were processed for cellular, biochemical, light and electron microscopic analysis after 4 and 24 h. Zymosan peritonitis provoked the expected signs of inflammation at 4 h, including a significant increase in extravasated PMNs in the mesentery and peritoneal exudate, mirrored by blood neutrophilia. These changes subsided after 24 h. Ultrastructural immunocytochemical analysis of PMNs showed significant Gal-1 expression and co-localization with L-selectin and beta 2-integrin in the plasma membrane and cytoplasm. Pharmacological treatment with hrGal-1 at 4 h produced an inhibition of PMN migration, associated with diminished expression of adhesion molecules, particularly beta 2-integrin, and TNF-alpha and IL1 beta release by peritoneal cells. At 24 h, Gal-1 induced an increase in mononuclear phagocytic cell recruitment. In conclusion, our data propose an important mechanism of anti-inflammatory action of Gal-1, initially by modulation of proinflammatory cytokine release and PMN migration through an imbalance between adhesion molecule expression and, later, by promoting monocyte-macrophage recruitment. (AU)

• Processo FAPESP: 08/00557-7 - Efeito do tratamento farmacológico com as proteínas anexina-A1 e galectina-1 na migração dos leucócitos durante uma inflamação aguda experimental
• Beneficiário: Caio Eduardo Gullo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica