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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of exogenous galectin-1 on leukocyte migration: modulation of cytokine levels and adhesion molecules

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Author(s):
Gil, Cristiane D. [1, 2] ; Gullo, Caio E. [2] ; Oliani, Sonia M. [3]
Total Authors: 3
Affiliation:
[1] Fed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, BR-04023900 Sao Paulo - Brazil
[2] Sao Jose do Rio Preto Sch Med FAMERP, Dept Anat, Sao Jose Do Rio Preto, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY; v. 4, n. 1, p. 74-84, 2011.
Web of Science Citations: 22
Abstract

The effect of exogenous Gal-1 on cellular response and adhesion molecule expression was investigated in a classical model of acute inflammation induced by zymosan. C57BL6 mice, treated or not with human recombinant (hr) Gal-1, received i.p. injection of zymosan and peritoneal exudate, blood and mesentery were processed for cellular, biochemical, light and electron microscopic analysis after 4 and 24 h. Zymosan peritonitis provoked the expected signs of inflammation at 4 h, including a significant increase in extravasated PMNs in the mesentery and peritoneal exudate, mirrored by blood neutrophilia. These changes subsided after 24 h. Ultrastructural immunocytochemical analysis of PMNs showed significant Gal-1 expression and co-localization with L-selectin and beta 2-integrin in the plasma membrane and cytoplasm. Pharmacological treatment with hrGal-1 at 4 h produced an inhibition of PMN migration, associated with diminished expression of adhesion molecules, particularly beta 2-integrin, and TNF-alpha and IL1 beta release by peritoneal cells. At 24 h, Gal-1 induced an increase in mononuclear phagocytic cell recruitment. In conclusion, our data propose an important mechanism of anti-inflammatory action of Gal-1, initially by modulation of proinflammatory cytokine release and PMN migration through an imbalance between adhesion molecule expression and, later, by promoting monocyte-macrophage recruitment. (AU)

FAPESP's process: 08/00557-7 - Effect of pharmacological treatment with annexin A1 and galectin-1 proteins in leukocyte transmigration during acute experimental inflammation
Grantee:Caio Eduardo Gullo
Support Opportunities: Scholarships in Brazil - Scientific Initiation