Relationships between gene polymorphisms of folate-related proteins and vitamins and metabolites in pregnant women and neonates

Autor(es)

Lopreato, Fernanda R. ^[1] ; Stabler, Sally P. ^[2] ; Carvalho, Felipe R. ^[1] ; Hirata, Rosario D. C. ^[1] ; Hirata, Mario H. ^[1] ; Robi, Dbora L. ^[1] ; Sarnpaio-Neto, Luiz F. ^[3] ; Allen, Robert H. ^[2] ; Guerra-Shinohara, Elvira M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Chem & Toxicol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Colorado, Hlth Sci Ctr, Dept Med, Denver, CO 80262 - USA

^[3] Pontificia Univ Catolica Sao Paulo, Fac Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinica Chimica Acta; v. 398, n. 1-2, p. 134-139, DEC 2008.
• Citações Web of Science: 8

Resumo

Background: The methylenetetrahydrofolate reductase (MTHFR), glutamate carboxypeptidase II (GCPII) and reduced folate carrier (RFC1) gene polymorphisms were associated with folate status. We investigated the effects of these polymorphisms on serum folate (SF) and folate-related metabolites in mothers and their neonates. Methods: Cobalamin (Cbl), SF, total homocysteine (tHcy), methylmalonic acid (MMA), S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH) were measured in 275 healthy women and their neonates. MTHFR C677T, GCPII C1561T and RFC1 A80G polymorphisms were determined by PCR-RFLP. Results: Maternal tHcy was affected individually by MTHFR C677T and GCPII C1561T polymorphisms and by combined genotypes MTHFR 677TT/GCPII 1561CC and MTHFR 677TT/RFC1 80AG. The MTHFR and RFC1 polymorphisms were not associated with variations in vitamins or SAM, SAH and MMA in neonates. Neonatal tHcy was predicted directly by maternal tHcy and inversely by maternal SF, neonatal Cbl and neonatal RFC1 80G allele (AG+GG genotypes). Maternal MMA and SAM/SAH were predicted by creatinine and Cbl, respectively. Neonatal MMA was predicted by maternal MMA and GCPII 1561T allele (CT+TT genotypes) and by neonatal Cbl. Conclusions: Maternal tHcy was affected by MTHFR C677T, RFC1 A80G and GCPII C1561T polymorphisms. Maternal GCPII C1561T variant was associated with neonatal MMA. Neonatal RFC1 A80G polymorphism influenced tHcy in neonates. (C) 2008 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/00740-5 - Influência dos polimorfismos nos genes TC2, RFC1 e GCP2 no metabolismo da homocisteína e nas reações de metilação em gestantes e em seus recém-nascidos
• Beneficiário: Elvira Maria Guerra Shinohara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/09836-7 - Analise das alteracoes em genes envolvidos no metabolismo das cobalaminas em gestantes, recem-nascidos e em individuos com deficiencia de cobalaminas.
• Beneficiário: Elvira Maria Guerra Shinohara
• Modalidade de apoio: Auxílio à Pesquisa - Regular