Jararhagin, a snake venom metalloprotease-disintegrin, activates the Rac1 GTPase and stimulates neurite outgrowth in neuroblastoma cells

Autor(es)

Costa, Erica Pereira ^[1] ; Del Debbio, Carolina Beltrame ^[1] ; Cintra, Leandro Carrijo ^[2] ; Costa, Luciano da Fontoura ^[2] ; Hamassaki, Dania Emi ^[1] ; Santos, Marinilce Fagundes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 52, n. 2, p. 380-384, AUG 1 2008.
• Citações Web of Science: 3

Resumo

It has been shown previously that the snake venom metalloprotease-disintegrin jararhagin stimulates cell migration and cytoskeletal rearrangement, independently of its effects on cellular adhesion but possibly associated with the activation of small GTP-binding proteins from the Rho family {[}Costa, E.P., Santos, M.F., 2004. Toxicon 44(8), 861-870.] Here we show that jararhagin stimulates spreading, actin dynamics and neurite outgrowth in neuroblastoma cells, and that this effect is accompanied by the translocation of the Rac1 small GTPase to the membrane fraction, suggesting its activation. Stimulation of neurite outgrowth was observed within minutes and was dependent on the proteolytic activity of the toxin. These results suggest that jararhagin may stimulate neuronal differentiation, being potential tool for neuronal regeneration studies. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/57508-2 - Mecanismos de sinalização envolvidos na hiperglicemia crônica: efeitos sobre a migração celular
• Beneficiário: Marinilce Fagundes dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/00587-5 - Modelagem por redes (grafos) e técnicas de reconhecimento de padrões: estrutura, dinâmica e aplicações
• Beneficiário: Roberto Marcondes Cesar Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 01/09047-2 - Desenvolvimento e degeneração da retina de vertebrados: aspectos celulares e moleculares
• Beneficiário: Dania Emi Hamassaki
• Modalidade de apoio: Auxílio à Pesquisa - Temático