Hemopressins and other hemoglobin-derived peptides in mouse brain: comparison between brain, blood, and heart peptidome and regulation in Cpefat/fat mice

Autor(es)

Gelman, Julia S. ^[1] ; Sironi, Juan ^[2] ; Castro, Leandro M. ^[3] ; Ferro, Emer S. ^[3] ; Fricker, Lloyd D. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Albert Einstein Coll Med, Dominic P Purpura Dept Neurosci, Bronx, NY 10461 - USA

^[2] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461 - USA

^[3] Univ Sao Paulo, Dept Cell Biol & Dev, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neurochemistry; v. 113, n. 4, p. 871-880, MAY 2010.
• Citações Web of Science: 38

Resumo

P>Many hemoglobin-derived peptides are present in mouse brain, and several of these have bioactive properties including the hemopressins, a related series of peptides that bind to cannabinoid CB1 receptors. Although hemoglobin is a major component of red blood cells, it is also present in neurons and glia. To examine whether the hemoglobin-derived peptides in brain are similar to those present in blood and heart, we used a peptidomics approach involving mass spectrometry. Many hemoglobin-derived peptides are found only in brain and not in blood, whereas all hemoglobin-derived peptides found in heart were also seen in blood. Thus, it is likely that the majority of the hemoglobin-derived peptides detected in brain are produced from brain hemoglobin and not erythrocytes. We also examined if the hemopressins and other major hemoglobin-derived peptides were regulated in the Cpefat/fat mouse; previously these mice were reported to have elevated levels of several hemoglobin-derived peptides. Many, but not all of the hemoglobin-derived peptides were elevated in several brain regions of the Cpefat/fat mouse. Taken together, these findings suggest that the post-translational processing of alpha and beta hemoglobin into the hemopressins, as well as other peptides, is up-regulated in some but not all Cpefat/fat mouse brain regions. (AU)

• Processo FAPESP: 04/14846-0 - Rede de proteoma do estado de São Paulo
• Beneficiário: Fabio Cesar Gozzo
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA
• Processo FAPESP: 04/04933-2 - Biologia celular molecular de oligopeptidases
• Beneficiário: Emer Suavinho Ferro
• Modalidade de apoio: Auxílio à Pesquisa - Temático