Allosteric Control of Gating Mechanisms Revisited: The Large Conductance Ca2+-Activated K+ Channel

Autor(es)

Rosales, Rafael A. ^[1] ; Varanda, Wamberto A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Math & Phys, BR-14049 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Physiol, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOPHYSICAL JOURNAL; v. 96, n. 10, p. 3987-3996, MAY 20 2009.
• Citações Web of Science: 2

Resumo

Large-conductance Ca2+-activated K+ channels (BK) play a fundamental role in modulating membrane potential in many cell types. The gating of BK channels and its modulation by Ca2+ and voltage has been the subject of intensive research over almost three decades, yielding several of the most complicated kinetic mechanisms ever proposed. A large number of open and closed states disposed, respectively, in two planes, named tiers, characterize these mechanisms. Transitions between states in the same plane are cooperative and modulated by Ca2+. Transitions across planes are highly concerted and voltage-dependent. Here we reexamine the validity of the two-tiered hypothesis by restricting attention to the modulation by Ca2+. Large single channel data sets at five Ca2+ concentrations were simultaneously analyzed from a Bayesian perspective by using hidden Markov models and Markov-chain Monte Carlo stochastic integration techniques. Our results support a dramatic reduction in model complexity, favoring a simple mechanism derived from the Monod-Wyman-Changeux allosteric model for homotetramers, able to explain the Ca2+ modulation of the gating process. This model differs from the standard Monod-Wyman-Changeux scheme in that one distinguishes when two Ca2+ ions are bound to adjacent or diagonal subunits of the tetramer. (AU)

• Processo FAPESP: 05/02063-3 - Estimação do gerador infinitesimal da dinâmica de canais iônicos
• Beneficiário: Rafael Andres Rosales Mitrowsky
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/50954-7 - Correntes iônicas e receptores na fisiologia de células excitáveis e não excitáveis
• Beneficiário: Wamberto Antonio Varanda
• Modalidade de apoio: Auxílio à Pesquisa - Temático