Structural and kinetic studies of Schistosoma mansoni adenylate kinases

Autor(es)

Marques, Ivo de Almeida ^[1] ; Romanello, Larissa ^[2] ; DeMarco, Ricardo ^[2] ; Pereira, Humberto D'Muniz ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Goias, Inst Fis, BR-74001970 Goiania, Go - Brazil

^[2] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Biochemical Parasitology; v. 185, n. 2, p. 157-160, OCT 2012.
• Citações Web of Science: 10

Resumo

The human parasite Schistosoma mansoni is totally dependent on the purine salvage pathway in order to supply large quantities of purine precursors for its energy and DNA biosynthetic needs. Adenylate kinase (ADK) is responsible for the conversion of AMP (produced by the adenosine kinase reaction) into ADP, which is subsequently converted into ATP by nucleoside diphosphate kinase (NDPK). ADK and NDPK are the most active enzymes of the pathway, probably reflecting an evolutionary adaptation due to the intense use of the branch in which they participate. However, notwithstanding their importance very little information has been accumulated found regarding these enzymes. In this work two adenylate kinases from S. mansoni were cloned and heterologously expressed in Escherichia coil. The purified products were utilized in activity assays, and displayed kinetic parameters similar to the corresponding human orthologous proteins. The cytosolic S. mansoni ADK was crystallized and its structure solved allowing us to detect a difference in the nucleotide binding site when compared with the human ortholog. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/60280-3 - Ensaios de inibição enzimática e cristalografia de alvos protéicos de parasitas tropicais
• Beneficiário: Humberto D'Muniz Pereira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 98/14138-2 - Center for Structural Molecular Biotechnology
• Beneficiário: Glaucius Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs