Enzymatic de-glycosylation of rutin improves its antioxidant and antiproliferative activities

Autor(es)

Melo Branco de Araujo, Maria Elisa ^[1] ; Moreira Franco, Yollanda E. ^[1] ; Alberto, Thiago Grando ^[1] ; Sobreiro, Mariana Alves ^[1] ; Conrado, Marco Aurelio ^[1] ; Priolli, Denise Goncalves ^[1] ; Frankland Sawaya, Alexandra C. H. ^[2] ; Ruiz, Ana Lucia T. G. ^[3] ; de Carvalho, Joao Ernesto ^[3] ; Carvalho, Patricia de Oliveira ^[1]

Afiliação do(s) autor(es)

^[1] USF, Lab Multidisciplinary Res, BR-12916900 Braganca Paulista, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Plant Biol, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, BR-13083970 Paulinia, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 141, n. 1, p. 266-273, NOV 1 2013.
• Citações Web of Science: 40

Resumo

Bioavailability and biological properties of flavonoid glycosides can be improved after the enzymatic hydrolysis of specific glycosyl groups. In this study, we evaluate the antioxidant and antiproliferative potential of rutin after enzymatic hydrolysis performed by alpha-L-rhamnosidases (hesperidinase from Penicillium sp. and naringinase from Penicillium decumbens) previously heated at 70 degrees C for 30 min to inactivate the undesirable beta-D-glucosidase activity. The highest in vitro antioxidant activity determined by DPPH radical scavenging was achieved with rutin hydrolyzed by hesperidinase. Rutin was predominantly bioconverted into quercetin-3-glucoside. There was no statistical difference between xanthine oxidase inhibition by rutin before and after hydrolysis. However, in vitro inhibitory activity against ten human tumor cell lines showed that hydrolyzed rutin exerted a more potent antiproliferative effect than quercetin and rutin on various cancer cell lines, specially glioma, and ovarian and breast adenocarcinomas. These results indicate that quercetin-3-glucoside could be a promising functional derivative obtained by rutin hydrolysis. (c) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
• Beneficiário: Paulo Mazzafera
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 11/12394-8 - Avaliação in vitro da atividade antioxidante e antimutagênica de flavonóides glicosilados e de suas agliconas
• Beneficiário: Mariana Alves Sobreiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/09224-3 - Biotransformação de polifenóis e potencial antioxidante
• Beneficiário: Patrícia de Oliveira Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular