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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Global gene expression in the bovine corpus luteum is altered after stimulatory and superovulatory treatments

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Autor(es):
Fatima, Luciana A. [1] ; Baruselli, Pietro S. [2] ; Gimenes, Lindsay U. [2] ; Binelli, Mario [2] ; Renno, Francisco P. [3] ; Murphy, Bruce D. [4] ; Papa, Paula C. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sect Anat, BR-05508270 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, BR-13635900 Pirassununga, SP - Brazil
[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Nutr & Anim Prod, BR-13635900 Pirassununga, SP - Brazil
[4] Univ Montreal, Anim Reprod Res Ctr CRRA, St Hyacinthe, PQ J2S 7C6 - Canada
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 25, n. 7, p. 998-1011, 2013.
Citações Web of Science: 12
Resumo

Equine chorionic gonadotrophin (eCG) has been widely used in superovulation and artificial insemination programmes and usually promotes an increase in corpus luteum (CL) volume and stimulates progesterone production. Therefore, to identify eCG-regulated genes in the bovine CL, the transcriptome was evaluated by microarray analysis and the expression of selected genes was validated by qPCR and western blot. Eighteen Nelore crossbred cows were divided into control (n = 5), stimulated (n = 6) and superovulated groups (n = 7). Ovulation was synchronised using a progesterone device-based protocol. Stimulated animals received 400 IU of eCG at device removal and superovulated animals received 2000 IU of eCG 4 days prior. Corpora lutea were collected 7 days after gonadotrophin-releasing hormone administration. Overall, 242 transcripts were upregulated and 111 transcripts were downregulated in stimulated cows (P <= 0.05) and 111 were upregulated and 113 downregulated in superovulated cows compared to the control animals (1.5-fold, P <= 0.05). Among the differentially expressed genes, many were involved in lipid biosynthesis and progesterone production, such as PPARG, STAR, prolactin receptors and follistatin. In conclusion, eCG modulates gene expression differently depending on the treatment, i.e. stimulatory or superovulatory. Our data contribute to the understanding of the pathways involved in increased progesterone levels observed after eCG treatment. (AU)

Processo FAPESP: 08/58839-8 - Analise diferencial da expressao genica e proteica no corpo luteo de bovinos submetidos a tratamentos com ecg.
Beneficiário:Luciana Alves de Fátima
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 08/58837-5 - Estimulacao de crescimento folicular com ecg e sua influencia em aspectos morfofuncionais das celulas luteinicas bovinas
Beneficiário:Paula de Carvalho Papa Keohane
Linha de fomento: Auxílio à Pesquisa - Regular