Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPAR alpha-dependent manner

Autor(es):Mostrar menos -

Bolsoni-Lopes, Andressa ^[1] ; Festuccia, William T. ^[1] ; Farias, Talita S. M. ^[1] ; Chimin, Patricia ^[1] ; Torres-Leal, Francisco L. ^[1] ; Derogis, Priscilla B. M. ^[2] ; de Andrade, Paula B. ^[1] ; Miyamoto, Sayuri ^[2] ; Lima, Fabio B. ^[1] ; Curi, Rui ^[1] ; Alonso-Vale, Maria Isabel C. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, BR-09913030 Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM; v. 305, n. 9, p. E1093-E1102, NOV 2013.
• Citações Web of Science: 25

Resumo

We investigated whether palmitoleic acid, a fatty acid that enhances whole body glucose disposal and suppresses hepatic steatosis, modulates triacylglycerol (TAG) metabolism in adipocytes. For this, both differentiated 3T3-L1 cells treated with either palmitoleic acid (16: 1n7, 200 mu M) or palmitic acid (16: 0, 200 mu M) for 24 h and primary adipocytes from wild-type or PPAR alpha-deficient mice treated with 16: 1n7 (300 mg.kg (-1.)day (-1)) or oleic acid (18: 1n9, 300 mg.kg (-1).day (-1)) by gavage for 10 days were evaluated for lipolysis, TAG, and glycerol 3-phosphate synthesis and gene and protein expression profile. Treatment of differentiated 3T3-L1 cells with 16:1n7, but not 16: 0, increased basal and isoproterenol-stimulated lipolysis, mRNA levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and protein content of ATGL and pSer(660)-HSL. Such increase in lipolysis induced by 16: 1n7, which can be prevented by pharmacological inhibition of PPAR alpha, was associated with higher rates of PPAR alpha binding to DNA. In contrast to lipolysis, both 16: 1n7 and 16: 0 increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose without affecting glyceroneogenesis and glycerokinase expression. Corroborating in vitro findings, treatment of wild-type but not PPAR alpha-deficient mice with 16: 1n7 increased primary adipocyte basal and stimulated lipolysis and ATGL and HSL mRNA levels. In contrast to lipolysis, however, 16: 1n7 treatment increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose in both wild-type and PPAR alpha-deficient mice. In conclusion, palmitoleic acid increases adipocyte lipolysis and lipases by a mechanism that requires a functional PPAR alpha. (AU)

• Processo FAPESP: 10/10909-8 - Envolvimento do tecido adiposo no desenvolvimento da obesidade e patologias associadas: investigação dos mecanismos moleculares e busca de novas alternativas terapêuticas
• Beneficiário: William Tadeu Lara Festuccia
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 09/53964-1 - Efeitos do acido palmitoleico na captacao e metabolismo de glicose e triacilglicerol em adipocitos brancos
• Beneficiário: Andressa Bolsoni Lopes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/51627-8 - Efeito de ácidos graxos sobre a diferenciação e função do tecido adiposo
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 09/15354-7 - Envolvimento do tecido adiposo no desenvolvimento da obesidade e patologias associadas: investigação dos mecanismos moleculares e busca de novas alternativas terapêuticas
• Beneficiário: William Tadeu Lara Festuccia
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores